Banca de DEFESA: CAROLINE CUNHA CARREIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CAROLINE CUNHA CARREIRO
DATA : 31/07/2018
HORA: 10:30
LOCAL: Instituto de veterinaris
TÍTULO:

LABORATORY DIAGNOSIS OF OPPORTUNIST PARABASALIDEOS IN DOMESTIC ANIMAL FACE SAMPLES

PALAVRAS-CHAVES:

Trichomonose, P. hominis, Tetratrichomonas sp; Small ruminants; swine

PÁGINAS: 146
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Patologia Animal
ESPECIALIDADE: Patologia Clínica Animal
RESUMO:

Trichomoniasis is caused by protozoa belonging to the phylum Parabasalia, which are found parasitizing the digestive tract and genitourinary of different animal species, including humans. Previously, these parabasalids were not considered to be responsible for emerging infections because they were involved not only in specific infection regions, but also in host-specific regions. However, it has been observed to the presence of a same protozoan with a great diversity of hosts, being these attributed to different clinical disorders, which indicates that these organisms are opportunistic and multiply when finding favorable conditions. At the site of infection they generate local inflammation and facilitate infection by other pathogens, and this synergism may intensify pathologies. In swine, these flagellates are described in the intestinal tract, and are associated with diarrhea; in the nasal cavity, is associated with atrophic rhinitis, in addition to other respiratory disorders. However, little is known about the possible parabasalid species involved in the parasitism of domestic animals, especially in small ruminants. The objective of this study was to diagnose the parabasalid species found in the gastrointestinal tract of goats, sheep and swine. For this, this study was divided in two parts. In the first one the frequency of these protozoa was evaluated in the small ruminants; the isolated flagellates were cultured and the kinetics of growth in culture medium was analyzed, as well as the morphological and molecular characteristics of these. The second part consisted in the molecular diagnosis of the isolated specimens of thirty five pigs in 2014, whose frequency and behavior in culture medium had already been evaluated previously. The frequency of parabasalis found was 42.85% in goats (6/14) and 68% in sheep (30/44), and the rate of co-infection between these fagelates and the other intestinal parasites was 42.85% in goats and 41.93% in sheep. Morphologically the strains presented similar characteristics to the genus Tetratrichomonas, and the sequencing showed that all strains analyzed presented 100% similarities with Tetratrichomonas sp isolated from bull prepuce. In pigs, in addition to diagnosing different species of the genus Tetratrichomonas, Pentatrichomonas hominis was still isolated. These flagellates are involved in several clinical disorders in different hosts, and the role of these animals as parabasalid reservoirs should be cautiously considered.

MEMBROS DA BANCA:
Presidente - 1201559 - VERA LUCIA TEIXEIRA DE JESUS
Interno - 6387102 - MARILENE DE FARIAS BRITO QUEIROZ
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Externo ao Programa - 387340 - CARLOS WILSON GOMES LOPES
Externo à Instituição - Janaina Barcelos Porto Ferreira - FIOCRUZ
Externo à Instituição - Isabella de Moura Folhadella Pires - FIOCRUZ
Externo à Instituição - SERGIAN VIANNA CARDOZO - Unigranrio