Banca de DEFESA: ANDRE ROMERO HENRIQUE DA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDRE ROMERO HENRIQUE DA COSTA
DATE: 22/12/2020
TIME: 13:30
LOCAL: Jitsi meet
TITLE:

Evaluation of the anti-inflammatory and antinociceptive activities of the essential oil of Myrciaria tenella O. Berg. 

KEY WORDS:

Myrciaria tenella, cytokines, inflammatory hypernociception, antiedematous effect, mice.

PAGES: 89
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Farmacologia e Terapêutica Animal
SUMMARY:

Currently, the main objective of research related to the treatment of pain and inflammatory, conditions related to a high degree of morbidity and mortality in the veterinary clinical routine, has been the development of new substances with greater efficiency, less toxicity and less economic cost than the drugs currently available, and the compounds extracted from medicinal plants, especially from aromatic plants, have aroused notable interest of researchers, regarding their chemical aspects and potential pharmacological properties. The aim of the present study was to evaluate the action of the essential oil of Myrciaria tenella leaves (OETM) on acute pain and inflammation, in vivo models, in Swiss mice, and in cell culture in vitro, in addition to the evaluation of cytotoxicity, in order to deepen the knowledge about its properties, mechanisms and toxicity. For in vivo biological assays, the OEMT was administered orally to male Swiss mice, showing action against chemically induced nociception (models of abdominal contortions induced by acetic acid and formalin) and against mechanically induced nociception (Von Frey model), where the reduction in the concentration of the pro-inflammatory cytokines IL-6, TNF-α and IL-1β and PGE2, in addition to the increase in the anti-inflammatory cytokine IL-10 accounted for the increase in the nociceptive threshold shown by the OEMT in the Von Frey model. OEMT did not induce motor damage or sedation in animals, confirming its antinociceptive action. The anti-inflammatory effect of OEMT was verified by its anti-edematogenic effect in the model of paw edema induced by carrageenan, but not in histamine or serotonin-induced edema, and by the reduction in the production of IL-6, TNF-α and NO in macrophage culture, in addition to inhibiting the enzymatic activity of both isoforms of COX (COX-1 and COX-2), showing selectivity for COX-1. OEMT did not produce toxicity in macrophage and fibroblast culture. In summary, OETM demonstrated anti-inflammatory effect in vivo and in vitro on different inflammatory mediators, accounting for its antinociceptive activity, in addition to demonstrating safety at the cellular level.

BANKING MEMBERS:
Interno - 1674073 - BRUNO GUIMARAES MARINHO
Externo ao Programa - 3000663 - DAVID DO CARMO MALVAR
Externo à Instituição - LUIS FELIPE SOUZA DA SILVA - UFS