Banca de QUALIFICAÇÃO: CAROLINE MUNIZ CUNHA RODRIGUES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAROLINE MUNIZ CUNHA RODRIGUES
DATA : 16/12/2018
HORA: 00:00
LOCAL: Instituto de Veterinária
TÍTULO:

Metabolic Response to Dietary Manipulation Pre-test in Equines Submitted to Incremental Speed Test

PALAVRAS-CHAVES:

Horses; Sport; Metabolism; Physical exertion test

PÁGINAS: 15
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
ESPECIALIDADE: Clínica Veterinária
RESUMO:

This study aims to evaluate the time of diet supply and / or type of diet (complete extruded diet or pelleted concentrate) provided pre-tests may directly influence blood glucose and insulin levels during physical exercise testing and performance of the horse athlete. The research will be conducted at the Equine Performance Evaluation Laboratory (LADEq / UFRRJ), located at the Army Riding School (EsEqEx) and at the EQUILAB Equine Health Research Laboratory located at the Veterinary Institute of the Federal Rural University of Rio de Janeiro of January. The experimental design will be completely randomized, in a factorial scheme with three treatments (pre-test diet schedule), two groups (diets), twelve repetitions (equines), evaluated in two incremental tests of speed. The two diets used will be: complete extruded diet (Nutratta Forrage Horse) and control diet (pelleted concentrate and coast-cross hay). Diets (complete extruded diet and pelleted concentrate) will be provided 2.5 h, 5 h, and 7.5 h prior to physical exercise testing. Twenty-four male or female equines, weighing an average of 500 kg, ranging in age from 8 to 14 years old, from the Brazilian Equestrian Race of the Army Riding School of the state of Rio de Janeiro, will compete in the modality of CCE in physical activity daily training. The animals will be submitted to clinical examination (physical examination and laboratory tests) and, being healthy, will be selected for the experiment. Equines will use cardiac frequencies (Polar Equine) to monitor heart rate. Equines will be adapted to exercise on a Galloper (Sahinco ®) high - speed treadmill and subjected to the tests. The test protocol will have a standardized 10 minute warm-up phase, 4 minutes at a rate of 1.7 m / s and 6 minutes at a 4.0 m / s speed. The speed increase phase will be performed with the treadmill at 3% incline to simulate the presence of the rider and will be composed of 1 minute at each incremental speed of gallop, at speeds of 6, 7, 8, 9 and 10m / s. After the tests, the deceleration of the gallops will be standardized by resuming the trot at a speed of 4.0 m / s for 3 minutes and, at a speed of 1.7 m / s for 2 minutes, followed by 25 minutes for the guide corresponding to the active cooling. Blood samples will be collected prior to diet delivery (Zero time); prior to the commencement of physical exertion tests; immediately upon completion of the tests; at 5, 10, 20, 30 and 60 minutes and at 6, 12, 24 and 36 hours after the tests. Before performing the exercise, the animals will be aseptically prepared for venocatterization using the left jugular vein as the collection point, at which point an extensor tube will be attached to the intravenous catheter to facilitate harvesting with the animal in motion. Blood samples for the analysis of lactate and glucose will be obtained in vacuum tubes with sodium fluoride while the blood samples will be collected in vacuum tubes without anticoagulant. In the blood samples the following analyzes will be carried out: Hemogram: which will be performed with the aid of an ABC-VET automatic counter. The specific leucometry and erythrocyte morphology analysis will be carried out under an optical microscope (100X), after staining of the smears by the Panótico Rápido. The analysis of fibrinogen and plasma protein will be in refractometer. Biochemical variables: will be used as exercise stress markers and the analyzes will be performed in a spectrophotometer (BTS 310): AST; CK; LDH. Hemogasometry: will be used in I-STAT (Roche ®) and EG7 + cartridges. The blood samples will be collected simultaneously to blood samples for blood counts and biochemical analyzes before and after the end of the stress test, later analyzed for the following variables: hydrogen-ionic potential (pH), carbon dioxide pressure (pCO2), oxygen pressure (pO2), bicarbonate concentration (HCO3) and plasma electrolytes. Lactate dosing: will be performed after separation and processing of the blood in 1.0 Ml Eppendorf tubes, using enzymatic methodology, with the aid of commercial reagent kit for lactate (Lactate K084-2, Bioclin) and using as described above spectrophotometer model BTS 310 (Byosistem®). Plasma insulin dosage will be made in duplicate by serum insulin reactivity previously determined by Radioimmunoassay (Coat-A-Count Insulin, Diagnostic Products, Los Angeles, CA). The results will be submitted to the Lilliefors test to verify normality, and the Cochran and Bartlett test to verify homogeneity of variance. The results of the variables will be submitted to analysis of variance and analyzed in a sub-divided plot scheme, the treatments (time of diet supply) being evaluated in the plots, the diets will be evaluated in the subplot and the physical effort tests in the sub -sub-plots. The averages will be compared by the Tukey test, at a 5% level of significance.

MEMBROS DA BANCA:
Interno - 2572430 - CRISTIANE DIVAN BALDANI
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Interno - 2193086 - MAGDA ALVES DE MEDEIROS
Interno - 2181722 - MARCELO ABIDU FIGUEIREDO