Risk factors and serology associated with Toxoplasma gondii and Neospora caninum infection in felines from the Itaguaí microregion of the State of Rio de Janeiro
RIFI, cats, coccidians
Toxoplasmosis is a cosmopolitan zoonosis caused by the protozoan Toxoplasma gondii and transmitted by the ingestion of oocysts present in the feces of infected cats, which are the definitive hosts of this parasite. When in the environment, these sporulated oocysts can reach the water of streams, lakes and wells destined for irrigation of crops and water supply of human and animal consumption, being able to be a source of infection for the population. In Brazil the prevalence in humans and animals is high, between 40% and 90% of pregnant women present the chronic form of the disease. Neosporosis is caused by the coccyx Neospora caninum, which has in the dog its definitive host. It is a disease widely studied in production animals such as cattle, goats and sheep, but little studied and almost unknown in domestic cats. The serum prevalence of T. gondii and N. caninum is related to low socioeconomic status and poor hygienic-sanitary conditions. The objective of this study is to correlate risk factors, clinical and laboratory aspects that predispose to infection in the feline population of the Itaguaí microregion, in order to establish seroprevalence in this population. The analyzes will be performed from 200 to blood samples of cats from the micro region of Itaguaí, attended in the feline sector of the Veterinary Hospital of Small Animals of the Federal Rural University of Rio de Janeiro. The serology will be done in the Laboratory of Coccidia and Coccidiosis (LCC) of the UFRRJ, through Indirect Immunofluorescence (IFR). Serum blood samples from the positive felines will be analyzed in the Laboratory of Clinical Pathology of the UFRRJ, regarding the serum activity of the enzymes: Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Gamma glutamyl transferase (GGT), Creatine Phosphorus kinase (CPK) and Alkaline Phosphatase (FA), in addition to total Protein (PT), Albumin, Urea and Creatinine concentrations. To quantitatively compare the population of positive animals with the associated risk factors will be used the chi-square test. It is hoped that this study will contribute to the epidemiological aspects of both parasites in the studied region, besides bringing valuable information in the clinical routine of felines.