Banca de QUALIFICAÇÃO: NATÁLIA BRAZ DE ALMEIDA PEREIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : NATÁLIA BRAZ DE ALMEIDA PEREIRA
DATA : 31/10/2018
HORA: 14:11
LOCAL: Seropédica
TÍTULO:

Immunohistochemical Diagnosis of Rabies in Dogs in the "Setor de Anatomia Patológica" of the "Universidade Federal Rural do Rio de Janeiro" (1953-2018)

PALAVRAS-CHAVES:

dog, central nervous system, encephalitis, rabies, immunohistochemistry, Lyssavirus.

PÁGINAS: 8
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Patologia Animal
ESPECIALIDADE: Anatomia Patologia Animal
RESUMO:

This study will be carried out at the Setor de Anatomia Patológica of the Universidade Federal Rural do Rio de Janeiro (SAP/UFRRJ), through the clinico-epidemiological and pathological data of rabies in dogs over seven decades (1953-2018). We postulate that the rabies in dogs decreased its incidence due to vaccination. The present study aims to gather information that may contribute to the understanding and prophylaxis of canine rabies, since there are no long term studies on rabies in dogs in Brazil. Formalin-fixed paraffin-embedded (FFPE) brain tissue obtained from necropsy of 352 dogs with encephalitis will be used to perform the immunohistochemistry (IHC) technique. Laboratory techniques are essential for the diagnosis of rabies. In Brazil, the official techniques recognized by the National Rabies Control Program in Herbivores of the Ministry of Agriculture for the diagnosis of rabies are direct immunofluorescence (IFD) and intracerebral inoculation in mice. In the histopathological examination, rabies can be recognized by the observation of lymphoplasmacytic meningoencephalitis with intraneuronal Negri bodies, considered the histological characteristic lesion of rabies. In cases where no inclusion bodies are observed, IHC can be used for the detection of viral antigen in FFPE tissue. It is an extremely valuable tool in retrospective studies in which refrigerated tissues are no longer available or in situations where no fresh brain samples were collected because rabies was not initially suspected. Obtain the epidemiological data from canine rabies from archived samples along this 65-year study, compare the IHC test with the diagnostic tests used in the past to diagnose rabies in dogs, and map the distribution of the antigen in different segments of the brain in rabid dogs will bring valuable information about the prevalence of the canine rabies. The region of brain with the highest amount of rabies antigen in domestic dogs could serve as a reference for sampling of brain tissue in wild carnivores. Results from this project may subsidize health surveillance actions in future cases.

MEMBROS DA BANCA:
Interno - 2932766 - ANDRESSA FERREIRA DA SILVA
Interno - 6387102 - MARILENE DE FARIAS BRITO QUEIROZ
Interno - 2929022 - SAULO ANDRADE CALDAS
Externo à Instituição - CAROLINE ARGENTA PESCADOR - UFMT
Externo à Instituição - PEDRO MIGUEL OCAMPOS PEDROSO - UnB