Banca de QUALIFICAÇÃO: RITA DE CÁSSIA LIMA MORAIS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : RITA DE CÁSSIA LIMA MORAIS
DATE: 27/01/2021
TIME: 08:00
LOCAL: Reprodução Animal - DRAA - IZ - UFRRJ
TITLE:

MICROBIOLOGICAL CHARACTERIZATION AND UTERINE VASCULAR REACTION PRE AND AFTER INTRAUTERINE USE OF DIMETHYLSULPHOXIDE FOR TREATING MARES WITH ENDOMETRITIS

KEY WORDS:

DMSO; Power Doppler; Ultrasound; Pregnancy.

PAGES: 212
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

The objective of the referred project was to evaluate the effectiveness of the intrauterine use of DMSO in mares with clinical / subclinical endometritis, characterizing its action in the inflammatory reaction, present microbiota and uterine vascularization. Forty-three mares were accompanied by two estrus (C1 and C2), performing Power Doppler uterine ultrasonography (UPD) after observation of a follicle ≥ 35mm and endometrial edema ≥ 3 (day 1 - D1) until one day before ovulation. On the D1 of each cycle, uterine samples were collected for cytological and microbiological examination. After the cytological examination, the following groups were distributed: G1 (n = 10): negative cytology and intrauterine DMSO administration; G2 (n = 10): negative cytology and administration of saline solution; G3 (n = 13): positive cytology and administration of DMSO and G4 (n = 10): positive cytology and administration of saline solution. According to each experimental group, the following protocol was entitled - D1: intrauterine infusion of 100 mL of DMSO 30% or sol. physiological; D2: intrauterine washing with 1000 mL of 30% DMSO or sun. physiological and 10 IU oxytocin, i.m., after the procedure. The protocol was repeated in C2, however, only in the sun. intrauterine physiological therapy was used. In subsequent cycles, the animals were inseminated or received embryos to assess fertility. In C2, G4 had a higher number of neutrophils / field than G1, G2 and G3 (p <0.05), while in the evaluation between cycles, in C2, G2 increased and G3 showed a reduction (p <0.05). For number of bacterial genera, in C1 and C2, G4 showed greater diversity than G1, G2 and G3 (p <0.05). Between cycles, in C2, there was a reduction only in G1 (p <0.05). As for the number of fungal genera, in C1, G1 did not show growth and G2, G3 and G4, were similar (p> 0.05). In C2, G4 had a higher number of genders than G2 and G3 (p <0.05). Between cycles, in C2, G2 and G3, the number of genders decreased (p <0.05) and G4 did not change (p> 0.05). In assessing fertility, G1, G2 and G3 had similar confirmed pregnancies with rates higher than G4 (p <0.05). In the intensity of the colored pixels (IPC) measured in the uterine UPD, it was observed: in C1, that G1 and G2 had CPIs lower than G3 and G4 (p <0.05) and G3 was greater than G4 (p <0.05). In C2, the CPI was higher in G4 (p <0.05). In the general average of the mares that became pregnant or not, regardless of the experimental group, between C1 and C2, it was observed that the empty mares maintained the CPI pattern (p> 0.05) and those that gestated showed a reduction in C2 ( p <0.05). It was concluded that the use of the uterine infusion / lavage protocol with DMSO eliminated or altered the microbiological load, decreased the inflammatory process favoring an increase in the fertility rate depending on the microorganism present and that the UPD can be used as an auxiliary diagnostic method in cases endometritis

BANKING MEMBERS:
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Interno - 1548043 - MARCO ROBERTO BOURG DE MELLO
Interno - 2929022 - SAULO ANDRADE CALDAS
Externo à Instituição - ALINE EMERIM PINNA - UFF
Externo à Instituição - GUSTAVO MENDES GOMES - UV
Externo à Instituição - MARCUS ANDRE FERREIRA SA