INDUCTION OF DOUBLE OVULATION IN SUB FERTILITY MARES
Key words: Equine. Biotechnology. Ovulation rate
One of the strategies to increase the efficiency of the embryo transfer program in mares is the induction of multiple ovulations. In this context the objectives of the present study is to verify the best protocol for induction of double ovulation, ovulation rate and embryo recovery in the studied groups. 50 stray cycles of 10 mares will be evaluated and they will be randomly distributed in the cross-over model. All animals will be induced to estrus on the 8th day of estrus cycle (D8). In experiment 1 the cycles will be divided into five groups: Group I (n = 10) treatment with 100 μg deslorelin every 12 hours when at least two nearby follicles of size between 15 and 20 mm are identified; Group II (n = 10) treatment with 100 μg of histrelin every 12 hours when at least two nearby follicles of size between 15 and 20 mm are identified; Group III (n = 10) treatment with 100 μg of deslorelin every 12 hours when at least two nearby follicles of size between 15 and 20 mm are identified, if there is a follicle of size much above the others, this should be aspirated before reaching the deviation (22.5 mm), always maintaining at least two follicles in size; Group IV (n = 10) treatment with 100 μg of histrelin every 12 hours when at least two nearby follicles of size between 15 and 20 mm are identified, if there is a follicle of size much above the others, this should be aspirated before reaching the deviation (22.5 mm), always maintaining at least two follicles of approximate size; GV (n = 10) will be the control group. In experiment 2 the protocol with the best result will be applied in a group of subfertile mares to evaluate their effectiveness. Statistical analysis will be performed using Fisher's exact test.