DEVELOPMENT OF PROTOCOL FOR SELECTIVE COLLECTIONS OF NERVOUS TISSUE SAMPLES IN BRAIN OF CATTLE AND HORSES
Neuroanatomy, neuropathology, histopathology.
Production animals affected by diseases with neurological signs must be necropsied and collections of fragments of the nervous system must be sent for histopathological examination, according to official guidance manuals. With regard to brain collection, it is important to standardize procedures and, at the same time, simplify protocols, seeking to meet the reality of the field professional, who often performs necropsies in difficult conditions, both in terms of geographic location and material resources. This work is expected to contribute to the production of a script for the selective collection of samples of nervous tissue with the set of nuclei at the base of the brain, diencephalon and limbic structures. The script will consist of images and short explanatory texts. The hypothesis is to test targeted collections that result in resource savings in the processing of material from the nervous system with a corresponding increase in the accuracy of morphological descriptions. Twenty bovine brains and twenty equine brains will be used to carry out this work. Plastic cassettes for histological processing will be positioned in the possible cleavage areas of the material, for recording the results in which more functional structures are accommodated per cassette, which will result in more diversified histological slides in the morphological aspect. Twenty equine and twenty bovine brains will be used to carry out this study.