Banca de DEFESA: OTÁVIA REIS E SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : OTÁVIA REIS E SILVA
DATE: 24/06/2022
TIME: 09:00
LOCAL: Encontro remoto via plataforma Google Meet
TITLE:

Identification of Pentatrichomonas hominis in naturally infected zebu cattle.

KEY WORDS:

Cattle, reproductive diseases. flagellated protozoa.

PAGES: 58
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

The aim of the present work is report the first presence of the flagellated protozoan
Pentatrichomonas hominis naturally parasitizing the reproductive tract of bovine species in
Brazil. Therefore, the tesis was divided into three chapes, the first captar referis to the report of
P. hominis in preputial wash of Nelore bulls, for the diagnosis samples of vaginal mucus and
preputial washes were collected in Lactopep medium and later cultivated in vitro in half Hanks,
being peaked every 72hrs. The culture samples that showed suspicion of the presence of mobile
organisms were submitted to molecular analysis using the polymerase chain reaction technique
and, for final confirmation of the diagnosis, another technique known as polymorphism in the
length of the restriction fragment and sequencing was used. The second chapter compares two
techniques for collecting vaginal mucus, one commonly used and recommended by most
current guides for collecting biological material and the other using the cytological brush,
widely used in human medicine and in equine cytology, for example, in this chapter, through
qualitative evaluations and cell culture, we can conclude that the use of the cytological brush
brings the benefit of being able to collect a greater amount of biological material, which can
facilitate and increase the chances of diagnosing parabasalids. The last chapter of the thesis is
an evaluation of the health profile of the farm and its influence on reproductive rates, for this,
a sampling was carried out on the farm animals and material was collected for serological
monitoring of diseases related to reproduction and still, these data were associated with
reproductive indices during two breeding seasons.

BANKING MEMBERS:
Presidente - 1548043 - MARCO ROBERTO BOURG DE MELLO
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Externo ao Programa - 1354903 - DOUGLAS MCINTOSH
Externa à Instituição - CLAUDIA DEL FAVA - USP
Externa à Instituição - MÁRCIA CRISTINA MENDES