Banca de QUALIFICAÇÃO: MARIANA PALHA DE BRITO JARDIM

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIANA PALHA DE BRITO JARDIM
DATE: 22/12/2022
TIME: 08:00
LOCAL: Instituto de Veterinária
TITLE:

Feline histoplasmosis in Rio de Janeiro - Clinical characterization and antigenic analysis

KEY WORDS:

Histoplasma, Gatos, EIA

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

Histoplasmosis is a systemic mycosis caused by the dimorphic fungus Histoplasma capsulatum. Diagnosis of the disease is commonly performed by cytology, histopathology or fungal culture, although these are linked to the need for invasive sample collection. The antigenic enzyme immunoassay (EIA) for Histoplasma can be performed on the serum and/or urine of the animals, although its use in feline medicine requires further investigation, since there are no studies that evaluate the effectiveness of antigenuria and antigenemia. Furthermore, few cases of histoplasmosis have been reported in cats in Brazil, which may be linked to underdiagnosis of the disease. The objective of this work is to characterize clinical data of felines with histoplasmosis, as well as to analyze the antigenic enzyme immunoassay for Histoplasma in the urine and serum of these animals, based on the previous diagnosis of such mycosis. Twelve domestic felines diagnosed with histoplasmosis by cytology, histopathology, mycology, Polymerase Chain Reaction (PCR), or therapeutic response were selected. Clinical, laboratory and imaging data were collected from these animals, as well as serum and/or urine samples. for performing serum and urinary EIA for Histoplasma, urinary Lateral Flow Test (LFA) for Histoplasma and analysis of serum antibodies of the IgG class for Histoplasma. Of the 12 animals with blood available for antigenic analysis, 11/91.67% had antigenemia and of the 11 cats in which urine could be collected, 9/81.82% had urinary antigens, so that all animals (100%) had serum or urine antigens. Of the seven animals evaluated for LFA 5/71.43% they were positive, as well as the eight feline samples submitted to analysis of IgG antibodies 5/62.5% were positive. Animals with mycosis did not differ significantly in terms of gender, although mixed breed and domiciled cats were more likely to have the condition. The mean age of sick cats was 6.75 years. Respiratory disorders were the predominant complaint reported by owners of cats with histoplasmosis, and dyspnea was the major clinical sign. Regarding laboratory alterations, lymphocytopenia and hypoalbuminemia were overrepresented. Pneumonia was the most common imaging finding, present in 12/100% of cats, with a higher occurrence of alveolar interstitial infiltrate, another frequent imaging alteration was hepatomegaly (8/66.67%). The diagnosis of histoplasmosis was made by lung cytology in 5/41.67% of the cases, histopathology in 4/33.33%, response to therapy in 2/16.67% of the animals, while skin cytology, PCR and mycology were employed once each (8.33%). The form of presentation of the fungal disease was mainly disseminated, followed by the pulmonary form. All animals (12/100%) of the research were therapeutically approached with antifungals, among which Itraconazole and Amphotericin B were the most used, where the applications of Amphotericin B were associated with an increase in serum creatinine. Corticosteroid therapy, if instituted before starting treatment with antifungals, was linked to negative clinical outcomes. The prognosis of the evaluated felines was mainly unfavorable, since 7/58.3% evolved to death or euthanasia.

COMMITTEE MEMBERS:
Presidente - 386998 - HELOISA JUSTEN MOREIRA DE SOUZA
Interno - 1151827 - BRUNO RICARDO SOARES ALBERIGI DA SILVA
Interno - 386849 - FRANCISCO DE ASSIS BARONI
Interno - 4563410 - JULIO ISRAEL FERNANDES