Banca de DEFESA: ANA CAROLINA DE SOUZA CAMPOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA DE SOUZA CAMPOS
DATE: 13/07/2023
TIME: 09:00
LOCAL: sala multimídia do LQEPV
TITLE:

Histopathological, immunohistochemical and ultrastructural analyzes of healthy omentum in bitches and cats. 

KEY WORDS:

Reconstructive surgery; healing; omentum; mylky spots; tissue repair

PAGES: 105
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Cirúrgica Animal
SUMMARY:

The omentum is a tissue present in all mammals and there is clear evidence of its properties in the treatment of animals, such as stimulating neovascularization, tissue reconstitution, lymphatic drainage, filling defects and increasing healing, even in sites with infection. Its application occurs in peritoneal and extraperitoneal lesions with wide use in surgery, but there is scarce information regarding its microscopic analysis and composition, not being found in Veterinary Medicine studies that compare the characteristics of the omentum between domestic species. It is necessary to study the components of the omentum in animals to understand the true value of this tissue, exploring its basic structure, to observe if there are clusters of immune cells, the known milk spots (MS), as reported in the human omentum, and to determine the composition and activity of this immune population. For this, omentum fragment samples measuring 2cm x 2cm were collected from 15 cats and 13 bitches during elective ovariohysterectomy surgery. The samples were, depending on the analysis carried out, divided into two, one fixed in 10% buffered formalin for the histopathology and immunohistochemistry technique to be performed at the Immunohistochemistry Laboratory of the Pathological Anatomy Sector of the Federal Rural University of Rio de Janeiro in order to detect immune cells (B and T lymphocytes, macrophages and dendritic cells); the other sample was fixed in a karnovizk solution for ultrastructural analysis by means of scanning and transmission electron microscopy performed at the Souto-Padrón and Lins Multi-User Microscopy Unit Laboratory of Microbiology at the Federal University of Rio de Janeiro. In the histopathological and immunohistochemical evaluations, MS were not found, only aggregates of defense cells, mainly in the translucent region and next to the vessels. The immune population was mainly composed of macrophages, followed by B and T lymphocytes in bitches; in the cats there was a smaller number of defense cells, only macrophages were detected. In electronic microscopy, the presence of pores and lymphatic stomata on the surface of the omentum in both regions was noted. It was concluded that despite the differences in structure and composition of the omentum in female dogs and cats compared to the human species, the findings of this study demonstrate the role of this tissue in the defense mechanisms against intraabdominal disorders and the validity of its use in the repair of extraperitoneal injuries in the evaluated species.

COMMITTEE MEMBERS:
Presidente - 3247698 - TICIANA DO NASCIMENTO FRANCA
Interno - 3015985 - FELIPE FARIAS PEREIRA DA CAMARA BARROS
Interna - 6387102 - MARILENE DE FARIAS BRITO QUEIROZ
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externo à Instituição - TIAGO DA CUNHA PEIXOTO - UFBA
Externa à Instituição - CLARICE MACHADO DOS SANTOS - UFF
Externo à Instituição - NEY LUIS PIPPI - UFSM