Banca de DEFESA: JOÃO PAULO NASCIMENTO ANDRADE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOÃO PAULO NASCIMENTO ANDRADE
DATA : 25/02/2019
HORA: 14:00
LOCAL: P1
TÍTULO:

SYNCHRONIZED INSEMINATIONS WITH A 21-DAY INTERVAL IN NELLORE HEIFERS AFTER EARLY DIAGNOSIS OF GESTATION WITH DOPPLER ULTRASONOGRAPHY.

PALAVRAS-CHAVES:

DOPPLER ULTRASONOGRAPHY, RESYNCHRONIZATION, BOVINE.

PÁGINAS: 22
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
ESPECIALIDADE: Inseminação Artificial Animal
RESUMO:

The objective of the study was to develop a new resynchronization program, adapted to the natural physiology of return heat, reducing the use of hormones and fixing treatments on the same days of the week. A total of 300 Nellore females weighing 291 kg were used. The heifers were synchronized for TAI (Timed Artificial Insemination), and the day of insemination was considered D0. The diagnosis of gestation (DG) was performed using a Mindray Doppler ultrasound, model Z5VET. The methodology for conducting the DG was based on the CL vascularization score (PUGLIESI et al., 2014). After the first conventional TAI (TAI-conv) the females were distributed in two resynch programs: PR-24 (inseminations with 24-day intervals) and PR-21 (inseminations with 21-day intervals). PR-24: D14 after TAI, heifers received an intravaginal progesterone (DI) device and intramuscular (im) application of 50mg long-acting injectable progesterone. At D22, the device was removed and immediately passed through Doppler DG. The females considered nonpregnant, received the application of 150μg of cloprostenol, 0.5mg of estradiol cypionate and 300UI of eCG (equine chorionic gonadotrophin) and were inseminated at D24. A replicatioon of this same treatment was carried out after the 2nd TAI ending the breeding season with 48 days. PR-21: D12 after TAI, heifers received only DI. At D19, the DI was removed and 300UI of eCG was applied. The DG Doppler was performed at D21, the females considered nonpregnant received the application (im) of 0.1mg gonadorelin (GnRH) and were inseminated immediately. A replication of this same treatment was performed after the 2nd TAI closing the season with 42 days. In resynchs, on day of AI, the size of the dominant follicle (FD) was verified. Ovulation rate (OT) and Doppler DG confirmation at the 2nd and 3rd resynch were performed after AIs at 12 and 11 days for PR-21; 14 and 11 for PR-24. The final DG was performed 40 days after the last AI of each program. False positive animals (FP) at the 1st Doppler DG passed for another TAI-conv. The variables were compared using the SAS program package. The conception rate (TC) of the first TAI-conv was 24.6% (74/300), resynch CTs were 33.6% (41/122) and 29% (45/155) respectively for PR-21 and PR-24. The TC of the TAI-conv performed in the females that were FP in the first DG was 21.6% (11/51), the pregnancy at the end of the season for PR-21 and PR-24 were 58.5% (86/147) and 55.6% (85/153). A difference was observed comparing resynch PR-21 + PR-24 31% (86/277) compared to the TAI-conv 24.6% (74/300) (P = 0.01). PR-21 heifers presented higher DF size compared to PR-24, 10.9 mm (± 1.9) vs. 10mm (± 2.1) respectively (P = 0.0003). No difference was observed for TO, 87% for PR-21 and 86.8% for PR-24. It was observed a difference between the bulls for conception 59.2% (29/49) vs 24.8% (130/525) (P = 0.0001) and incidence of DG FP 6.5% (2/31) vs. 34% (57/167) (P = 0.01).

MEMBROS DA BANCA:
Presidente - 1548043 - MARCO ROBERTO BOURG DE MELLO
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Interno - 1201559 - VERA LUCIA TEIXEIRA DE JESUS
Externo à Instituição - ALINE EMERIM PINNA - UFF
Externo à Instituição - JOAQUIM ESQUERDO FERREIRA - CESVA