Banca de QUALIFICAÇÃO: ISADORA PIRES FERREIRA DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ISADORA PIRES FERREIRA DOS SANTOS
DATE: 22/04/2024
TIME: 09:00
LOCAL: Area de Reprodução Animal (SFRIA)
TITLE:

Evaluation of pre-ovulatory follicle blood flow with different ovulation inducers

KEY WORDS:

Follicular control, Diagnosis, Doppler ultrasound

PAGES: 48
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

The use of ovulation-inducing agents plays a crucial role in anticipating the natural ovulation process to a predictable period of 48 hours. The aim of this study was to evaluate the use of different ovulation inducers to stimulate follicular vascularization. The study was carried out in the equine sector of the UFRRJ during the reproductive seasons of 2022/2023 and 2023/2024. A total of 54 estrous cycles were used. The selected mares were separated into four experimental groups according to the type of ovulation inducer used: CG - control group: mares that were not submitted to ovulation induction (13 estrous cycles); GhCG - group of mares induced with hCG (13 estrous cycles); GD - group of mares induced with deslorelin acetate (13 estrous cycles); GH - group of mares induced with histrelin acetate (15 estrous cycles). The study began by monitoring the estrous cycle until a pre-ovulatory follicle was observed (diameter ≥ 35mm and edema ≥ 3). The mares were then randomly assigned to the treatment groups, where they were either induced to ovulate or follicular monitoring was carried out until ovulation occurred spontaneously (control group). For the GhCG group, 1 ml with 1000 IU of hCG IV (Chorulon®5000 IU) was used; for the GD group, 3 ml with 750µg of deslorelin acetate IM (Sincrorrelin®) was used; and for the GH group, 1 ml with 250µg of histrelin acetate IM (Strelin®) was used. The moment of ovulation induction was considered to be the zero hour (0h) of the ultrasound evaluations, continuing every 12h until ovulation. The mares were examined by B-mode ultrasound (US) to assess uterine edema and follicular diameter, Power Flow Doppler US with subjective assessment of follicular vascularization and spectral Doppler US measuring the pulsatility (PI) and resistivity (RI) indices of blood flow in the ovarian artery of the ovarian pedicle ipsilateral to the pre-ovulatory follicle. Among the results obtained, there was a significant difference in uterine edema within each group at all the times assessed; in the assessment of follicular vascularization, there was a significant difference in the CG between 12 and 24 hours (p=0.0242) and in the GH between 0 and 36 hours (p=0.0227); in the assessment of ovarian artery vascularization, there was a significant difference in the IR of the GH between 12 and 36 hours (p=0.0465). When the groups were compared within each hour, no statistical difference was observed in the variables analyzed. There was also a tendency towards higher pregnancy rates when there was a decrease in the subjective assessment of follicular vascularization, PI and RI and when there was proportionality to uterine edema. It can therefore be concluded that there is no difference between the inducers in stimulating an increase in follicular vascularization during the ovulatory process, nor is there any influence on the pregnancy rate. However, the histrelin acetate-based inducer showed a decrease in follicular vascularization between the time of induction and ovulation, which makes it a good parameter for assessing possible complications during the ovulatory process.

COMMITTEE MEMBERS:
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Interno - 1548043 - MARCO ROBERTO BOURG DE MELLO
Externo à Instituição - ALINE EMERIM PINNA - UFF