Banca de DEFESA: BRUNA SAMPAIO MARTINS LAND MANIER

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNA SAMPAIO MARTINS LAND MANIER
DATA : 20/02/2020
HORA: 09:00
LOCAL: Laboratório de Quimioterapia Experimental em Parasitologia Veterinária
TÍTULO:

ISOLATION AND SUSTAINABILITY OF ANTIMICROBIANS OF ISOLATED DOGS SKIN BACTERIA WITH SECONDARY PIODERMITE TO CANINE ATOPIC DERMATITIS

PALAVRAS-CHAVES:

bacterial resistance, oxacillin, mec-A, recurrent pyoderma, dermatitis canine atopic.

PÁGINAS: 20
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
ESPECIALIDADE: Clínica Veterinária
RESUMO:

Pyoderma is defined by bacterial skin infection, whose main clinical signs are the presence of pustules, papules, scabs, epidermal vests and erythema. The main bacteria involved in pyoderma processes is Staphylococcus pseudintermedius. The diagnosis is based on cytological examination, but only bacterial culture followed by antibiogram allows the most appropriate therapeutic choice for treatment. Canine pyoderma occurs secondarily to another disease most of the time, with canine atopic dermatitis being the most associated underlying disease. Empirical use of antibiotics is a recurrent practice in the clinical routine in the treatment of atopic dogs with signs of pyoderma. This indiscriminate use of antibiotics has been related to the emergence of resistant and multiresistant bacteria, a problem that, besides being a challenge for the treatment of pyoderma, can become a public health problem, since the transmission of bacteria between canine and the human species has already been reported. The objective of this work will be to survey strains of resistant and multiresistant bacteria isolated in dogs with canine atopic dermatitis treated at the Veterinary Hospital of the Federal Rural University of Rio de Janeiro through antimicrobial susceptibility testing and identification of mec-A gene, responsible for conferring resistance to beta-lactam antibiotics. Fifty atopic dogs will be selected, meeting at least 5 of the 8 Favrot criteria and presenting clinical signs of pyoderma. Material will preferably be collected from pustules or epidermal vests with the aid of sterile swab. The material will be immediately taken to the Veterinary Bacteriology Laboratory (LabacVet) for isolation of bacterial strains. The identification of bacterial species will be performed by the polymerase chain reaction (PCR) technique. For antibiogram the strains of Staphylococcus spp. will be submitted to disk diffusion screening tests by sowing the isolates. Genotypic detection of methicillin resistance will be performed for both susceptible and beta-lactam resistant Staphylococcus strains by PCR for detection of mec-A gene. It is expected to report the occurrence of methicillin resistant bacteria, as well as the occurrence of multiresistant bacteria in atopic dogs treated at UFRRJ.

MEMBROS DA BANCA:
Interno - 4563410 - JULIO ISRAEL FERNANDES
Externo à Instituição - ARY ELIAS ABOUD DUTRA - UCB
Externa à Instituição - REGINA HELENA RUCKERT RAMADINHA