Banca de DEFESA: LUMARA RAELI LIGEIRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUMARA RAELI LIGEIRO
DATE: 12/03/2021
TIME: 14:00
LOCAL: Via google meet ou Zoom
TITLE:

Markers of neural damage in the blood of dogs with spinal cord injury caused by intervertebral disc disease

KEY WORDS:

Cerebrospinal fluid, intervertebral disc disease, spinal cord injury, dog

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

The spinal cord injury occurs due to traumas and diseases in several species, and affects in different degrees the ability of the spinal cord to send and receive information, generating sensory, motor and autonomic deficiencies. In dogs, spinal cord compression due to intervertebral disc disease is the main cause of spinal cord injury and clinical signs vary depending on the duration, location, degree of protrusion and chronicity of the disease. Thus, research on disc disease has focused on description, understanding and imaging complementary tests, as well as the analysis of the clinical conditions of these patients in order to improve the prognosis. Thus, the detection of neural damage markers may represent a strategy to assess the severity of neuronal damage, to relate to clinical signs and to establish a more accurate prognosis. A good marker of neural damage must be sensitive, have high specificity for neurological tissue and, if possible, be measured in serum or plasma, since CSF puncture is more laborious than blood collection and is not without risk. In medullary lesions, the initial severity of neurological impairment is the best predictor of long-term outcomes. To assess the extent of spinal cord injury in the acute phase there is a new approach by measuring specific neural proteins. The trauma leads to the release of metabolites and proteins from nerve tissue in cerebrospinal fluid and blood, such as calcium-binding protein S100 beta (S100β), neuron-specific enolase (NSE), neurofilament protein (NFL), myelin basic protein ), glial fibrillar protein (GFAP), monocyte chemotactic protein 1 (MCP_1), and interleukins 6 and 8 (IL-6 and IL-8) which are potential markers of spinal cord injury. The general objective of the study is to correlate the concentrations of markers: NSE, GFAP, S100β, NFL, IL-6 and MCP_1 present in the serum or plasma of dogs with different degrees of medullar compression caused by intervertebral disc protrusion or extrusion. 24 dogs, regardless of race, weight and sex, aged between 3 and 10 years, with a maximum of 10 days of injury, divided into two groups according to degree of spinal cord injury and a control group, will be classified according to the Olby and Frankel scales and submitted to blood collection for analysis of the biomarkers (quantified by the chemiluminescence technique or real-time PCR (qPCR).

BANKING MEMBERS:
Presidente - 2193086 - MAGDA ALVES DE MEDEIROS
Interna - 387386 - MARTA FERNANDA ALBUQUERQUE DA SILVA
Externo ao Programa - 387295 - LUIS CARLOS REIS
Externa à Instituição - ANGÉLICA DA SILVA VASCONCELLOS - PUCMinas
Externa à Instituição - LUCIENE COVOLAN - UNIFESP