Banca de DEFESA: MAURILIO ROSA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAURILIO ROSA
DATE: 10/06/2022
TIME: 14:00
LOCAL: Videoconferencia
TITLE:

IMMUNOHISTOCHEMICAL MORPHOLOGICAL CHARACTERIZATION OF BIOMATERIALS USING THE EQUINE AMNIOTIC MEMBRANE AS SUBSTRATE

KEY WORDS:

amniotic membrane, healing, wounds, cornea, immunohistochemistry, laminin, fibronectin, collagen I and IV

PAGES: 78
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Modern Medicine has sought in Bioengineering solutions for the most varied diseases that affect human and animal health. Bioengineering is an area of knowledge in development. The use of materials with low or no toxicity and immunogenicity becomes imperative. Equine Amniotic Membrane (EAM) is a good source of tissue and cells potentially capable of promoting both tissue and organ reconstruction. In addition, it has other biological properties important for tissue engineering, including anti-inflammatory, antimicrobial, antifibrosis, anti-healing quality, reasonable mechanical property, and low immunogenicity. It represents part of the universe of materials that are proposed for therapeutic use in areas such as orthopedics, dentistry, oncology and reconstructive surgery for burns. However, despite the large number of works already published studying the amniotic membrane, basic questions still need to be answered, as the mechanisms by which this biomaterial acts on organisms are still not completely elucidated. In this thesis, we established and validated protocols for the collection, preparation and preservation of the equine amniotic membrane that allow obtaining a viable biomaterial, free from contamination, for use as a medicine. We morphologically characterized by immunohistochemistry three different types of biomaterials from the MAE, the freeze-returned membrane (AMNn), the dehydrated membrane (AMNd) and the decellularized membrane (AMNTx), for the presence of bioactive components present in the biotissue and able to participate as facilitating agents in the healing process. The results obtained in the evaluation of each biomaterial developed, culminates in proof of the maintenance of components, laminin, fibronectin and collagen I and IV in the structure of most samples of the biomaterials studied, with the exception of the AMNd immunohistochemistry for collagen I which had inconclusive result. This information becomes extremely important, since these elements play an important role in promoting support, stimulating angiogenesis, controlling inflammation, cell migration and adhesion during the tissue healing process. The next step should be to implement the use and collection of data related to the clinical performance of each of these biomaterials.

BANKING MEMBERS:
Presidente - 2181699 - PAULO DE TARSO LANDGRAF BOTTEON
Interna - 387386 - MARTA FERNANDA ALBUQUERQUE DA SILVA
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externo ao Programa - 2396528 - LEONARDO RODRIGUES DE LIMA
Externo à Instituição - ALEXANDRE DE LIMA ANDRADE
Externo à Instituição - JORGE DA SILVA PEREIRA
Externa à Instituição - CLÁUDIA HELENA PELLIZZON