Banca de QUALIFICAÇÃO: MARIA EDUARDA DOS SANTOS LOPES FERNANDES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA EDUARDA DOS SANTOS LOPES FERNANDES
DATE: 21/09/2023
TIME: 13:30
LOCAL: INSTITUTO DE VETERINÁRIA
TITLE:

Influence of free omental graft without microvascular anastomosis on the integration of mesh skin grafts applied in experimental wounds in rabbits.

KEY WORDS:

Omentum; Oryctolagus cuniculus; Reconstructive Surgery; Wound Healing

PAGES: 76
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Cirúrgica Animal
SUMMARY:

In veterinary medicine, reconstructive surgeries are commonly performed in surgical clinical routines, with autologous skin grafts being widely employed to provide coverage for skin defects resulting from various causes. Since the skin graft does not maintain its vascularization at the time of implantation and requires preparation of the recipient bed, the outcome of this technique is not always satisfactory. Due to its angiogenic properties, vascular richness, and the presence of growth factors, the omentum has been studied and used to aid in wound healing, especially in the form of a pedicled graft. However, there have been no studies found on the use of free omental grafts without vascular microanastomosis to assist in the integration of mesh skin grafts. Therefore, the present study aims to evaluate the influence of free omental grafts without vascular microanastomosis on the implantation of mesh skin grafts applied to experimentally created open wounds in rabbits. Twenty-four rabbits from the Laboratory of Experimental Chemotherapy in Veterinary Parasitology at the Federal Rural University of Rio de Janeiro (UFRRJ) were used. A midline longitudinal celiotomy was performed to collect a 4cm² omental fragment from each rabbit. After celiorrhaphy, two square wounds of 4cm² each were created on both sides of the dorsal thoracic region of all animals. Each removed skin segment was prepared to serve as a mesh skin graft, applied to the contralateral wound. In the control wounds (CW), the skin graft was sutured to the recipient bed using simple interrupted stitches with 4-0 polyamide thread, without the presence of the omental fragment between the graft and the recipient bed. In the treated wounds (TW), the omental fragment was implanted into the recipient bed using four simple interrupted stitches with 4-0 polydioxanone thread at its ends, followed by suturing of the skin graft as described earlier. The wounds were macroscopically evaluated on postoperative days 3, 7, 10, 14, 17, 21, and 28, until biopsy for microscopic evaluation was performed. The animals were kept for periods of 7, 14, and 28 days (eight animals in each period) and were then euthanized to obtain material for histopathology. Clinically, there was an increase in volume that remained for a longer and more intense period, especially with a firm consistency on the sides of the grafts, starting on the third postoperative day in TW. Furthermore, there was a higher production of inflammatory exudate, which persisted for a longer period with faster progression to dark, desiccated, and devitalized appearances in TW when compared to CW during the evaluation periods. Macroscopic histopathological evaluation showed a more intense organic reaction in TW compared to CW at all evaluation time points, but these reactions eased and evolved toward tissue repair over time in both groups. Microscopy corroborated the macroscopic evaluation data, revealing increased inflammation and organic reaction with a higher number of polymorphonuclear cells, mononuclear cells, and giant cells on the 14th and 28th postoperative days, greater intensity of epidermal ulcers on the 28th day, and a lower reepithelialization rate on the 14th and 28th days in TW compared to CW.

COMMITTEE MEMBERS:
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Interno - 3015985 - FELIPE FARIAS PEREIRA DA CAMARA BARROS
Interna - 387386 - MARTA FERNANDA ALBUQUERQUE DA SILVA
Interna - ***.073.401-** - PAULA VELOZO LEAL - UFBA
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO