Banca de DEFESA: CINTHIA CRISTINA JARDIM

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CINTHIA CRISTINA JARDIM
DATA : 19/12/2019
HORA: 08:30
LOCAL: Area de Reprodução Animal - IZ - DRAA
TÍTULO:

EVALUATION OF DIFFERENT OVULATION INDUCTORS IN DIFFERENT AGES

PALAVRAS-CHAVES:

Mare, ovulation, Estral cycle

PÁGINAS: 35
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
ESPECIALIDADE: Fisiopatologia da Reprodução Animal
RESUMO:

The purpose of inducing ovulation is directly related to the best use of biotechniques such as artificial insemination (AI), embryo transfer (ET), follicular aspiration, which require precision at the time closest to ovulation. In addition, induction is also beneficial in mares that are inseminated with frozen, cooled semen and for synchronization of donors and recipients in an ET program. The present experiment will be conducted in private Haras, near the region of Seropédica, Rio de Janeiro, Brazil. It will be used 36 mares and 108 cycles of strains of varied races, presenting between. Only mares without reproductive abnormalities assessed by ultrasonography will participate in this study. The animals will be divided into groups by age, Gj = young (up to 10 years), Gm = mature (between 10 and 18 years) and Gv = old (above 18 years) and evaluated in different periods of the mating season October / November-December / January-February and March / April). The mares will have their reproductive tract evaluated daily, until the largest follicle reaches the diameter ≥35mm, as well as the evaluation of the endometrial edema for confirmation of estrus, where we will begin the treatments with 1000 IU of hCG (EV) (Chorulon®) and 1000 μg deslorelin acetate (IM) (Sincrorrelin®) and the association of both according to the groups: G1j = young mare application of 1,000 IU hCG (EV); G1m = mature mare application of 1,000UI of hCG (EV); G1v = old mare application of hCG (EV) 1000UI and G1C = control mares, 1ml of saline solution. G2j = young mare application of 1000 μg deslorelin (I.M.); G2m = mature mare application of 1000 μg deslorelin (I.M.); G2v = old mare application of 1000 μg deslorelin (I.M.); G2c = control mares, 1ml of saline solution. G3j = young mare application of 1,000 IU hCG (EV) + 1000 μg deslorelin acetate; G3m = mature mare application of 1,000 IU hCG (EV) + 1000 μg deslorelin acetate; G3v = Old mare application of hCG (EV) 1000UI + 1000 μg deslorelin acetate; G3c = control mares, 1ml of saline solution. Each treated group will have nine mares, all mares from each age group will go through all treatments randomly throughout the experiment. The control group will have only three mares in each age group and three cycles of each mare will be used, totaling nine cycles per group. Twenty four hours after treatment the mares will be monitored every 6 hours until ovulation is identified. For the statistical analysis of follicle data such as: follicle size at induction, time between induction and ovulation, pre ovulatory follicle diameter (6 hours before ovulation), and increased follicle diameter after induction, age of the groups and season, the ANOVA (Variance Analysis) method will be used, followed by the Tukey test when necessary. Data on the percentage of mares ovulated at ≤ 36 hours, 36 to 42 hours, 42 to 48 hours or ≥ 48 hours were analyzed using the chi-square (χ2) at a significance level of 5%.

MEMBROS DA BANCA:
Presidente - 2202309 - JULIO CESAR FERRAZ JACOB
Interna - 1201559 - VERA LUCIA TEIXEIRA DE JESUS
Externo à Instituição - GUSTAVO MENDES GOMES - UV