Banca de QUALIFICAÇÃO: ROSÂNGELA ANTUNES TERRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ROSÂNGELA ANTUNES TERRA
DATA : 01/10/2018
HORA: 09:00
LOCAL: Instituto de Veterinária sala 26
TÍTULO:

Intramammary application of platelet rich plasm for dry cow therapy

PALAVRAS-CHAVES:

immunology, bovine, mastitis

PÁGINAS: 73
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
ESPECIALIDADE: Clínica Veterinária
RESUMO:

Platelet Rich Plasma (PRP) has been successfully used in several fields of medicine with anti-inflammatory and antimicrobial properties and its in loco use may induce a local immune response in the bovine mammary gland and minimize antibiotics use. This work aimed to evaluate the response of PRP intramammary in dry cow therapy and its effect on subsequent lactation. Nine crossbred cows (holsteinxzebu), healthy, pregnant, reactive to California Mastitis Tests were used. One week prior to drying, blood samples (Sample 1 / A1) were taken for complete blood count and TBARS, and milk for microbial isolation, composition determination, somatic cell count, differential cell count and interleukin dosing. On the last day of milking, after emptying and antisepsis of the teats, treatments were instituted in intramammary applications: T1 (3 cows, 12 mammary quarters (QM)): antibiotic (ATB); T2 (3 cows, 12 QM): 10 mL PRP; T3 (3 cows, 12 QM): 10 mL of PRP with ATB. The analyzes were repeated between 24 and 36 hours postpartum (D0) and 14, 30 and 60 days after (D14, D30 and D60). The design was completely randomized with arrangement in subdivided plots. Data were submitted to variance analysis and the means were compared using Bonferroni test (Prism 5.0), with a confidence level of 95% (p≤0,05). The method of obtaining PRP was efficient, concentrating blood platelet counts almost 10 times (290 ± 40 for 2,477 ± 500 platelets / mL). There was no difference in CCS in A1 (p> 0.05), with high count in all groups. After delivery, the highest CCS mean was verified on D30 in T3, with a difference in relation to T1. CCS was lower in T1 at all times postpartum, intermediate in T2 (D14 and D60) and higher in T3 (D14 and D60). Greater CCS may indicate mobilization of defense cells to mammary gland, but indicative of inflammatory reaction. Regarding the composition of the milk, there were A1, with lower fat contents in T2 and the three groups differed in relation to the total dry extract, dry extract and proteins. In postpartum, the fat and ESD contents of the T2 were similar to the others, showing a recovery in milk production by the mammary gland. Regarding the hemogram, all constituents of the erythrogram at all times and groups remained within the normal range for the species. In white cells, leukocytosis was observed in T1 (between D0 and D30), T2 (D0) and T3 (all moments), with a predominance of left shift. In the samples collected before drying, ECN was the most isolated microorganism, followed by Streptococcus spp.. and Staphylococcus spp. After calving, regardless of the treatment, there was a predominance of ECN and Corynebacterium spp. Data from TBARS, interleukins and differential milk cell counts are needed to conclude the effects of PRP use at the time of drying.

MEMBROS DA BANCA:
Presidente - 387400 - RITA DE CASSIA CAMPBELL MACHADO BOTTEON
Externo ao Programa - 386994 - CARLOS HENRIQUE MACHADO
Externo ao Programa - 3701492 - HUARRISSON AZEVEDO SANTOS
Externo ao Programa - 1545840 - LUCIA HELENA PINTO DA SILVA