Banca de QUALIFICAÇÃO: BARTOLOMEU BENEDITO NEVES DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : BARTOLOMEU BENEDITO NEVES DOS SANTOS
DATA : 22/11/2018
HORA: 13:00
LOCAL: Auditório do Anexo I do Instituto de Veterinária
TÍTULO:

Immunophenotyping and Clonality Study by PCR for Detection of Rearrangement of Antigen Receptor Genes in Extramedullary Plasmocytomas and Canine Cutaneous Histiocytomas

PALAVRAS-CHAVES:

Cutaneous plasmacytoma, dog, dentritic cells

PÁGINAS: 12
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Patologia Animal
ESPECIALIDADE: Anatomia Patologia Animal
RESUMO:

Canine Extramedullary Plasmacytomas (CEP), mainly cutaneous, are frequent and represent about 1.5 to 2.3% of all skin neoplasms in dogs, however there are several aspects not clear in relation to CEP, especially with regard to epidemiology, morphological variations, pathogenesis and etiology. Currently, six different histological types of CEP are described in dogs, indicating that such neoplasia presents great morphological variation. On the other hand, many CEPs present morphological characteristics very similar to malignant neoplasms of histiocytic origin, with marked nuclear pleomorphism and multinucleation. However, the cellular spectrum encompassed by the histiocytic term is generic and includes both dendritic cells and macrophage lineage cells. Dendritic cells originate in the bone marrow from pluripotent hematopoietic stem cells and give rise to two cell lines: dendritic myeloid and dendritic lymphoid. It is relevant investigating whether CEPs will exhibit any different pattern in the immunostaining or clonality by PCR for the detection of antigen receptor gene rearrangement (PARR). This study aims to characterize CEPs and  canine histiocytomas by expression of cell markers and determination of clonality by PCR technique to PARR. This method will be used for differentiation of non-lymphoid versus lymphoid origin of tumors. Immunophenotypic characteristics will be verified in 100 CEPs and 30 cutaneous histiocytomas by anti-CD79a, CD20, CD3 and MUM-1 antibodies. CD138 antibody will also be tested as a possible alternative to MUM-1, since in according to recent literature these antibody to present cross-reaction with canine histiocytomas. The CD123 for the hypothesis of possible lymphoid dendritic origin of the CEPs. Thus, the immunohistochemical protocol used for the diagnosis of CEPs is expected to be acured, as well as to standardize and validate the PCR technique for the study of clonality in CEPs and to test the lymphoid versus non-lymphoid hypothesis of some CEPs (with a clonality study and immunostaining for plasmacytoid dendritic cells with CD 123), which may, in part, reveal the difference in behavior of CEPs, especially in relation to multiple myeloma.

MEMBROS DA BANCA:
Interno - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externo ao Programa - 3377607 - APARECIDA ALVES DO NASCIMENTO
Externo à Instituição - DAVID DRIEMEIER - UFRGS
Externo à Instituição - PEDRO SOARES BEZERRA JUNIOR - UFPA