Banca de DEFESA: ILKA MARIA DE SOUZA SILVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ILKA MARIA DE SOUZA SILVEIRA
DATE: 26/02/2021
TIME: 09:00
LOCAL: plataforma ggogle meet
TITLE:

Identification of parabasalids in feces of shelter, domiciled and stray cats in the municipality of Guapimirim, Rio de Janeiro, using two diagnostic methods.

KEY WORDS:

parabasalid, mixed-breed cats, domiciled, sheltered

PAGES: 59
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

Dogs and cats can host a wide range of parasites, which can affect both the health and well-being of these animals and that of humans. Tritrichomonas foetus and Pentatrichomonas foetus are flagellated protozoa, and in recent years have been identified as an agent of chronic diarrhea in cats, due to parasitism of the colon mucosa. The animal can remain a carrier for many years, with no clinical sign. Prevention is based on improving the environmental conditions of your habitat. This study aimed to investigate the existence of parabasalids in cat feces, located in the municipality of Guapimirim, State of Rio de Janeiro. For this purpose, cats from all over the city were used, without delimiting neighborhoods or districts, regardless of sex and age. To sensitize the owners and help to control endemic diseases in the city, a veterinarian in charge of the veterinary clinic Ilka Silveira, designed a castration campaign, involving cats, kept in shelters, domiciled and stray. On the day of castration, feces were collected from the rectal ampoule or by rectal lavage, from 200 felines. All animals were registered on a form with information about age, race, place of origin and whether symptoms occurred. The feces were transported in natura, at room temperature to perform the research of parabasalids. This material arrived at the laboratory in a maximum of six hours. From the cats collected, a sample of 30 cats was submitted to the OPG exam, having been collected in a separate pot and these feces cooled. In the laboratory, such samples were added with 0.89% saline solution for homogenization and subjected to optical microscopy examination, with 10x and 40x magnification. With the visualization or not of the parabasalids, the samples were added to the Hank culture medium, incubated at 35º ± 2ºC with growth evaluation every 24 hours, by direct examination under optical microscopy. Subsequently, the stool culture was centrifuged, and the precipitate was frozen and kept in the freezer for PCR examination. The DNA was extracted from the samples in culture and the rRNA genes were amplified by polymerase chain reaction (PCR), using universal primers (TFR1 and 2) and two other species specific to T. foetus (TFR3 and 4). As a result of this study, parabasalids were not identified in the samples collected, either by direct stool examination or by PCR. As for stool sampling for parasitological examination, Ancylostoma spp. and Dipylidium sp. As a result, we identified that crossbred cats, sheltered or not, have more resistance to this parasitosis.

BANKING MEMBERS:
Presidente - 1201559 - VERA LUCIA TEIXEIRA DE JESUS
Interno - 2202309 - JULIO CESAR FERRAZ JACOB
Externo ao Programa - 387340 - CARLOS WILSON GOMES LOPES
Externo à Instituição - MARCUS ANDRE FERREIRA SA - UES
Externo à Instituição - SERGIAN VIANNA CARDOZO - Unigranrio