Banca de DEFESA: RITA DE CÁSSIA LIMA MORAIS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RITA DE CÁSSIA LIMA MORAIS
DATE: 29/03/2021
TIME: 16:00
LOCAL: Reprodução Animal - DRAA - IZ - UFRRJ
TITLE:

Effect of intrauterine infusion of Dimethylsulfoxide on vascularization and the uterine microbiological profile in mares with endometritis

KEY WORDS:

DMSO; Power Doppler; Ultrasound; Pregnancy.

PAGES: 212
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

The objective of this study was to evaluate the efficacy of the intrauterine use of dimethyl sulfoxide (DMSO) in mares with clinical / subclinical endometritis, characterizing its action in the inflammatory reaction, present microbiota and uterine vascularization. Forty-three mares were accompanied by two oestrus (C1 and C2), performing Doppler uterine ultrasonography in power flow mode (UPF) after observation of a follicle ≥ 35mm and endometrial edema ≥ 3 (day 1 - M1) until one day before ovulation . In the M1 of each cycle, uterine samples were collected for cytological and microbiological examination. After the cytological examination, the following groups were distributed: G1 (n = 10): negative cytology and intrauterine administration of DMSO; G2 (n = 10): negative cytology and administration of saline solution; G3 (n = 13): positive cytology and administration of DMSO and G4 (n = 10): positive cytology and administration of saline solution. According to each experimental group, the following protocol was performed - M1: intrauterine infusion of 100 mL of 30% DMSO or physiological solution; M2: intrauterine washing with 1000 mL of 30% DMSO or saline and 10 IU oxytocin, i.m., after the procedure. The protocol was repeated in C2, however, only the intrauterine saline solution was used. In the third cycle, the animals were inseminated or received embryos to assess fertility. In C2, G4 had a higher number of neutrophils / field than G1, G2 and G3 (P <0.05), while in the evaluation between cycles, in C2, G2 increased and G3 showed a reduction (P <0.05). As for the number of bacterial genera, in C1 and C2, G4 showed greater diversity than G1, G2 and G3 (P <0.05). Between cycles, in C2, there was a reduction only in G1 (P <0.05). As for the number of fungal genera, in C1, G1 did not show growth and G2, G3 and G4, were similar (P> 0.05). In C2, G4 had a higher number of genders than G2 and G3 (p <0.05). Between cycles, in C2, G2 the number of genders decreased in G2 and G3 (p <0.05) and in G4 it did not change (p> 0.05). In the evaluation of fertility, G1, G2 and G3 had similar confirmed pregnancies with rates higher than G4 (P <0.05). In the intensity of the colored pixels (CPI), measured through uterine UPF, it was observed that: in C1, G1 and G2 had CPIs lower than G3 and G4 (P <0.05) and G3 was greater than G4 (P <0, 05). In C2, the CPI was higher in G4 (P <0.05). In the general average of mares that became pregnant or not, regardless of the experimental group, between C1 and C2, it was observed that non-pregnant mares maintained the CPI standard (P> 0.05) and those that gestated showed a reduction in C2 (p <0.05). It was concluded that the use of the DMSO infusion / uterine lavage protocol decreased the inflammatory process, eliminated or altered the microbiological load and increased fertility and that UPF can be used as an auxiliary diagnostic method in cases of clinical endometritis.

BANKING MEMBERS:
Presidente - 2202309 - JULIO CESAR FERRAZ JACOB
Interno - 1548043 - MARCO ROBERTO BOURG DE MELLO
Interno - 2929022 - SAULO ANDRADE CALDAS
Externo à Instituição - GUSTAVO MENDES GOMES - UV
Externo à Instituição - ALINE EMERIM PINNA - UFF