Banca de DEFESA: AGATHA FERREIRA XAVIER DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : AGATHA FERREIRA XAVIER DE OLIVEIRA
DATE: 29/11/2021
TIME: 09:00
LOCAL: webconferência
TITLE:

Research of infections by Anaplasmataceae, Pyroplasms and Hemoplasms agents in black-eared possums (Didelphis aurita) from the Metropolitan Region of Rio de Janeiro

KEY WORDS:
PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The occurrence of hemoparasitosis in marsupials is described worldwide in countries such as Brazil, Australia, United States, China, among others. Despite this, studies on black-eared opossums (Didelphis aurita) are scarce. The objective of this study was to investigate the presence of hemoparasites belonging to the Anaplasmataceae family, Pyroplasmas and also hemoplasms in peripheral blood and bone marrow, as well as the existence of co-infections in Didelphis aurita from the metropolitan region of Rio de Janeiro. A total of 15 black-eared opossums were received at the Centro de Triagem de Animais Silvestres – CETAS RJ and at the Centro de Reabilitação de Animais Silvestres – CRAS RJ. Upon appropriate restraint, the animals were clinically evaluated and inspected for the presence of ectoparasites. In addition, peripheral blood samples were collected through venipuncture of the tail vein and transferred to tubes containing the anticoagulant EDTA, to perform the blood count. Bone marrow collection was performed after sedation in only ten of the fifteen animals due to their adequate body score. To avoid false-negative results, all samples were subjected to prior molecular analysis to detect the endogenous genes gapdh and IRBP. DNA detections from Anaplasmataceae and Mycoplasma spp. were based on the 16S rRNA gene and Pyroplasmas on the 18S rRNA gene. All whole blood and bone marrow samples tested were negative for Anasplasmataceae Agents. 20% (3/15) of the animals were positive for Mycoplasma spp. only in whole blood samples and of these 66.6% (2/3) had inflammatory comorbidities. For the order Piroplasmid 33.3% (5/15) of the animals were positive in whole blood and bone marrow samples and 80% (4/5) had comorbidities. One animal positive for Pyroplasma presented icteric mucosa and decreased mean corpuscular hemoglobin value and another animal presented anemia (hematocrit of 23.5%) and decreased plasma protein value (5g/dl). 13.33% (2/15) of the opossums were co-infected with Pyroplasma and Hemoplasma in whole blood samples, and only one had comorbidity. The data from this study indicate the existence of important hemoparasite infections in Didelphis aurita from the metropolitan region of Rio de Janeiro, which reinforces the need for new epidemiological studies to clarify the participation of these animals in the occurrence of these diseases in domestic animals and humans.

BANKING MEMBERS:
Interna - 2572430 - CRISTIANE DIVAN BALDANI
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Externa ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Externa à Instituição - ANDRESA GUIMARÃES - UFRRJ
Externo à Instituição - MARCOS ROGERIO ANDRE - UNESP