Banca de DEFESA: NAIARA VIDAL STOCCO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NAIARA VIDAL STOCCO
DATE: 29/11/2021
TIME: 14:00
LOCAL: webconferência
TITLE:

Hematological and molecular study of infection by haemoporids and anaplasmataceae agents in blue-and-white macaw (Ara ararauna) and white-lined parrot (Amazona aestiva) kept at the wild animal screening center (CETAS-RJ)

KEY WORDS:

Psittacids, hemoparasites, hematology, cytochrome b, 16SrRNA

PAGES: 33
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Agents of the order Haemosporida and Rickettsiales can affect different groups of animals, including birds, and cause relevant consequences to animal and/or human health. Birds play an important role in the transmission of etiological agents, mainly due to their migration, nesting and feeding habits. IBAMA's Triage Centers house a considerable number of birds of different species. These individuals come from the apprehension of trafficking, or illegal creation and arrive constantly weakened in these institutions, being prone to diseases and the spread of pathogens. These conditions can present themselves in a subclinical manner, impacting suspicion and diagnosis and increasing their transmissibility, as well as clinically, which will depend on the host's immune response. In the present study, 44 birds, 19 Gray Macaws (Ara ararauna) and 25 Gray Parrots (Amazona aestiva) sampled from the Wild Animal Screening Center of Rio de Janeiro (CETAS), located in the municipality of Seropédica, were examined. Diagnostic methods (direct detection in blood smears, conventional PCR and phylogenetic analysis) were performed in order to verify the presence of agents of the Anaplasmataceae family (Ehrlichia spp., Anaplasma spp.) and Order Haemosporida (Plasmodium spp., Haemoproteus spp.). Only the macaws (Ara ararauna) were positive for the agents surveyed. In the evaluation of blood smears stained by Giemsa, structures compatible with hemoparasites were found in 2 (4.54%) birds, suggestive of Plasmodium spp./Haemoproteus spp. In the conventional PCR based on the 16SrRNA gene for Anaplasmataceae and on the cytochrome b gene for hemoporids, 3 (6.81%) individuals were positive, being 1 (2.27%) bird positive for the 16SrRNA gene and 2 (4.54%) for the cytochrome b gene. Among the positive individuals, one sample referring to the cytochrome b gene, was sequenced and the analysis of alignments and production of the phylogenetic tree was performed, demonstrating positivity for Plasmodium, closely related to the nucleophilum lineage, not registered so far in the Ara ararauna species. As for the results of the blood counts, although the hematological alterations presented by these animals may not have been conclusive, one infected individual presented leukocytosis (n=1; 33.33%), while another developed anemia associated with leukopenia (n=1; 33.33%). The other parameters did not differ from the literature. Although the study has shown a low prevalence of infection by these pathogens, the results highlight that birds from CETAS/RJ are exposed to the agents and can be affected by the infection, highlighting the diagnostic importance before the relocation of these individuals, in order to prevent transmission of these pathogens and impacts on fauna.

BANKING MEMBERS:
Interna - 2572430 - CRISTIANE DIVAN BALDANI
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Externa ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Externa à Instituição - ANDRESA GUIMARÃES - UFRRJ
Externo à Instituição - MARCOS ROGERIO ANDRE - UNESP