Banca de DEFESA: IURY UZEDA DA ROCHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : IURY UZEDA DA ROCHA
DATE: 23/09/2022
TIME: 13:30
LOCAL: https://meet.google.com/snp-veih-rty
TITLE:

Use of entomopathogenic fungi and essential oil of Illicium verum (Illiciaceae) in the in vitro control of Aedes aegypti.

KEY WORDS:

biological control, mosquitoes, Metarhizium anisopliae

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:

The present study evaluated the compatibility between the isolates of Beauveria bassiana (LCM S19 and LCM S20) and Metarhizium anisopliae (LCM S01) with the essential oil of Illicium verum, the effectiveness of the isolates and the oil separately in the control of larvae, pupae and adults. of Aedes aegypti and also the in silico analysis of the oil. For compatibility between fungi and oil, the germination of the isolates and the growth of colony diameter were evaluated. For the germination percentage, 10 μL of fungal suspension associated or not with oil were inoculated into plates containing BDA + 0.5% chloramphenicol. After 16 hours of incubation, the percentage of germination was verified. To evaluate radial colony growth, 10 μL of each suspension were inoculated in the center of plates containing Oat medium and measured for 9 days. For larvicidal and pupicidal activity, groups containing 10 larvae or pupae of A. aegypti were kept in disposable cups containing 15 mL of essential oil solution at 40 ppm, 60 ppm, 80 ppm and 100 ppm or fungal suspension at concentrations of 104, 105, 106, 107 and 108 propagule/ml. The survival of larvae and pupae was monitored daily. In the biological assay with adults, 10 mosquitoes were transferred to disposable cups containing filter paper, previously impregnated with 1mL of essential oil concentrations or fungal concentrations, covering the entire inner surface of the cup. The control group of all experiments contained dechlorinated water with 0.03% Tween 80. The data were submitted to the normality test, and after submitted to the Analysis of Variance using Tukey's test. The Kaplan-Meier and Log-rank tests were used to analyze the survival curve and the mean survival time (S50). The significance level was 95% (P≤0.05). The concentrations of I. verum OE did not interfere with conidia germination and did not change the colony growth of the isolates LCM S01 (Metarhizium anisopliae), LCM S19 and LCM S20 (Beauveria bassiana). In the biological assay with the O.E., it showed larvicidal activity at all concentrations studied and pupicidal activity at concentrations of 60 and 100 ppm. In silico analysis showed that 79.96% of the oil is composed of (E)-anethole that can affect 89 possible targets in the arthropod. In the biological assay with the fungal isolates, all, regardless of the propagule used, were able to reduce the survival rate of the larvae at a concentration of 108 propagule/mL. Pupicidal activity
VII
was demonstrated by all isolates at different concentrations. The LCM S01 isolate was able to reduce adult survival at three different concentrations. The isolates LCM S19 and LCM S20 were also virulent at the highest concentrations for adults. It is concluded that the fungal isolates and the essential oil are compatible and good options for controlling larvae, pupae and adults of A. aegypti.

BANKING MEMBERS:
Presidente - 2860277 - ISABELE DA COSTA ANGELO
Interna - 2221855 - PATRICIA SILVA GOLO
Externo à Instituição - EVERTON KORT KAMP - UFG
Externo à Instituição - RICARDO DE OLIVEIRA BARBOSA BITENCOURT
Externo à Instituição - WENDELL MARCELO DE SOUZA PERINOTTO - UFRB