Banca de DEFESA: EDUARDA DE OLIVEIRA SILVA LIMA MACHADO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : EDUARDA DE OLIVEIRA SILVA LIMA MACHADO
DATE: 03/03/2023
TIME: 09:00
LOCAL: Sala 23 do Departamento de Parasitologia Animal
TITLE:
Cytological, molecular, and epidemiological investigation of bacteria of the Mycoplasmataceae family in populations of small mammals in regions of the states of Rio de Janeiro and Parana
KEY WORDS:
Hemoplasma, hemotropic mycoplasmas, epicellular, bacteria.
PAGES: 61
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:
Hemotropic mycoplasmas, also known as hemoplasmas, are found in several animal species worldwide. New species and new genotypes of Mycoplasma have been described in wild animals. However, reports of hemotrophic mycoplasmas in small non-flying mammals in Brazil are scarce. Therefore, this study aimed to carry out a cytological analysis that involves aspects of morphology, morphometry, and parasitemia, in addition to molecular diagnosis and the genetic diversity of bacteria of the Mycoplasmataceae family in populations of small non-flying mammals in regions with different characteristics of the states of Rio de Janeiro and Parana. A total of 258 small mammals were captured. The cytological analysis was performed using a blood smear stained with Giemsa solution under optical microscopy. For the morphometric study, measurements of the length and width of the cocci were used using the CellSens software. Molecular detection of agents from the Mycoplasmataceae family was based on the 16S rRNA gene. The products amplified in the polymerase chain reaction (PCR) were selected and purified for subsequent sequencing and construction of the phylogenetic tree. Mycoplasma spp. amplified from Akodon paranaensis was joined to another 30 sequences, with Mycoplasmoides fastidiosum being used as an outgroup, in a dataset of 537 positions. Among the 258 samples of small wild mammals analyzed, 23.2% (n=60) presented structures compatible with Mycoplasma sp. in erythrocytes through cytological investigation. The bacteria were arranged in single cocci, diplococci, grouped or in chains along the surface of the erythrocytes. A statistically significant difference was observed in the mean length and width between Mycoplasma de Akodon spp. and the one found in Sooretamys angouya, with S. angouya cocci being considered more oversized. As for the location, there was a statistically significant difference between the averages of cocci width and parasitemia between Rio de Janeiro and Parana, with statistically more significant cocci width and parasitemia found in animals from Parana. Regarding molecular diagnosis, 33.7% (n=87) amplified Mycoplasma sp. in conventional PCR. The region with the highest frequency of positivity was Cruz Machado (46.15%, n = 24/52), followed by Ponta Grossa (43.10%, n=25/58), Nova Friburgo (30.56%, n= 33/108), and finally Lidianopolis (12.50%, n=5/40). Oligoryzomys had the highest percentage of positivity for Mycoplasma spp. (78.05%), statistically differing from Oxymycterus spp. (42.11%), Akodon spp. (27.59%) and Sooretamys (9.09%). Males were more frequently parasitized with Mycoplasma spp. than females (p<0.001). Regarding the phylogenetic analysis, the sequence of Mycoplasma spp. amplified from Akodon paranaensis showed 100% identity with Mycoplasma from Oligoryzomys nigripes from São Paulo and Minas Gerais, forming a clade with these sequences. This study revealed the morphological, morphometric, eco-epidemiological, and phylogenetic aspects of Mycoplasma spp. in small non-flying wild mammals in regions of the states of Parana and Rio de Janeiro, Brazil.
COMMITTEE MEMBERS:
Presidente - 3103478 - MARISTELA PECKLE PEIXOTO
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Interno - 3701492 - HUARRISSON AZEVEDO SANTOS
Externa à Instituição - MARIA LUCIA BARRETO - UFF
Externa à Instituição - NATHALIE COSTA DA CUNHA - UFF