Banca de DEFESA: INGRID LINS RAQUEL DE JESUS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : INGRID LINS RAQUEL DE JESUS
DATE: 09/03/2023
TIME: 14:00
LOCAL: Sala do Professor Laerte Grisi (LQEPV)
TITLE:

Development of topical pharmaceutical formulations containing eugenol and carvacrol for the control of ectoparasites in companion animals

KEY WORDS:

Ectoparasiticides, Natural products, Formulation design

PAGES: 123
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The increased proximity between humans and animals has led to increased concern about the health and well-being of these animals, such as the control of the flea Ctenocephalides felis felis and the tick Rhipicephalus sanguineus, which are parasites of great importance, whose control is carried out usually with synthetic products. With the increase in reports of resistance of parasites to treatment, natural products are an alternative with antiparasitic and less toxic activity. The aim of this work was to develop topical formulations containing bioactives to combat Ctenocephalides felis felis and Rhipicephalus sanguineus adults, to carry out their physical-chemical characterization and preliminary physical stability, to develop an analytical method for the detection and quantification of bioactives in the formulations and to evaluate their residual and knock down effect through in vitro bioassays. For this, formulations were developed in the form of spray and spot-on, which were submitted to tests of appearance, pH, centrifugation, freezing and heating cycle, quantification by high performance liquid chromatography coupled to the ultraviolet detector. The microemulsions were developed containing a mixture of surfactants, co-solvent and bioactive, left under agitation for a period of 2 hours. To carry out the in vitro bioassays, the filter paper impregnation method was used and in the evaluation of the knock down effect, the evaluations were carried out at times of 15, 30, 45 and 60 minutes, 2, 4, 6, 8 and 24 hours, analyzing the same individuals; for the residual efficacy, the analyzes were performed daily, until no more mortality was observed, replacing the individuals after each evaluation. The formulations were stable for a period of 60 days, with the necessary characteristics for topical application in dogs. The spray formulations showed acaricidal activity for 3 days, and the formulation containing eugenol and its association with carvacrol reached 100% mortality after 24 hours, reaching 10% after the 3^rd day; and the formulation containing carvacrol, started with 77% of activity, reaching 10% in the same period. As for the insecticidal activity of the spray formulations, they showed activity > 90% for 22 days for eugenol and its association with carvacrol and for 16 days for carvacrol; still showing activity after the 84^th day. The formulations in the form of spot-on, presented activity superior to 90% in the first 7 days of analysis, presenting activity inferior to 10% in the 43^rd day those containing isolated eugenol and carvacrol and of 17% and 20% for the spot-on formulations on with bioactives in association incorporated through a nanoemulsion and in the absence of an emulsified system. The evaluation of the knock down effect against fleas showed that the spray formulations containing carvacrol and eugenol reach the maximum activity in 4 hours, while the one that presents an association of bioactives takes approximately 2 hours, while the spot-on formulations showed 100% mortality between 30 and 45 minutes after the start of exposure. All the developed formulations presented the physicochemical characteristics suitable for topical application in pets and stability for 60 days, showing pulicidal and tickicidal activity with promising results for continuation of in vivo tests, after evaluating their safety and dermal irritation.

COMMITTEE MEMBERS:
Presidente - 1700427 - YARA PELUSO CID
Interno - ***.088.737-** - DIEFREY RIBEIRO CAMPOS - UFRRJ
Interna - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Externa ao Programa - 3304705 - ERIKA YOKO SUZUKI - UFRRJExterno ao Programa - 1809376 - LUIZ HENRIQUE GUERREIRO ROSADO - UFRRJExterna à Instituição - PATRÍCIA GARCIA FERREIRA - UFF