Banca de DEFESA: CRISTIANE DE MEDEIROS CAMPANHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CRISTIANE DE MEDEIROS CAMPANHA
DATE: 27/09/2023
TIME: 13:00
LOCAL: Sala 14 do Departamento de Parasitologia Animal
TITLE:

Molecular characterization of Trypanosoma sp. in wild birds at different altitude gradients in Itatiaia National Park, Brazil.

KEY WORDS:

Trypanosoma, Atlantic Forest, wild birds, Phylogenetic analysis

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The Atlantic Rainforest is one of the largest centers of biodiversity and species endemism in the world. Relief is one of the elements responsible for producing so much diversity. The environmental conditions in the mountains are very different from those in lowland areas because they have a greater temperature range, water restrictions and greater geographical isolation. As a result, species experience adaptations that reflect environmental variations and can disrupt biotic exchanges with neighboring plains. Hematozoan parasites such as Trypanosoma sp. are transmitted by a series of dipteran vectors abundantly present in this environment, affecting most local hosts and exerting selective pressure on avifauna, causing deleterious effects on health, reproductive success, bbehavior,and community structure. The aim of this study was to correlate the elevation gradient of Itatiaia National Park with the prevalence and diversity of Trypanosoma sp. in the blood of wild birds using blood smears and PCR screening to verify the predominance of this hematozoan. Of the 230 slide smears observed under microscopy, the infective evolutionary form of Trypanosoma was found in 17 and 42 samples were PCR positive. Prevalence in the lower elevation gradient, below 1,000, was higher than in the higher elevation gradient, above 2,000, which suggests that temperature, environmental conditions, and vector diversity are more abundant in lower elevation areas, favoring transmission of the hematozoan. Phylogenetic analyses revealed 17 new sequences very close to Trypanosoma Polygranularis found in Peliperdix lathami in Cameroon, Africa. A single sequence clustered close to the Trypanosoma everetti clade, a branch close to the Trypanosoma everetti-like species found in the host Dumetella carolinensis, USA. Our study demonstrated the diversity and prevalence of trypanosome species in different altitudinal gradients in the PNI. In Itatiaia National Park, the genetic diversity among Trypanosoma lineages in wild birds appears to be quite limited. Out of the 18 sequences obtained during this study, an impressive 17 are closely related to Trypanosoma polygranularis according to their phylogenetic analysis. Furthermore, the Trypanosoma sp. strains identified in this research exhibit a broad host range, as they have been observed in no fewer than 12 different bird species.

COMMITTEE MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Interno - 386163 - CARLOS LUIZ MASSARD
Interna - 3103478 - MARISTELA PECKLE PEIXOTO
Externa à Instituição - ALINE MOREIRA DE SOUZA - UFF
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ