Banca de DEFESA: OLIVIA ZEN GIANFRANCISCO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : OLIVIA ZEN GIANFRANCISCO
DATE: 28/09/2023
TIME: 13:30
LOCAL: Videoconferência
TITLE:

Molecular diagnosis of hemoparasites in the blood of non-human primates in captivity in the state of Rio de Janeiro

KEY WORDS:

Hemoparasitosis, Primates, Zoonoses, Trypanosoma, Bodo saltans.

PAGES: 64
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Brazil is the country with the largest number of known primates, and these animals, like other wildlife, have the potential to be hosts for a wide range of hemoparasites, including zoonotic agents. The Atlantic Forest biome, which encompasses the state of Rio de Janeiro, is the second largest one in terms of primate species diversity, and these animals, under increasing anthropization, have been interacting more frequently with the human population. The purpose of this study was to detect species of various hemoparasites through molecular analysis of one hundred and seventy-eight blood samples from primates of different species kept in captivity in the state of Rio de Janeiro, monitoring and identifying the occurrence of such agents in these animals. The sources of the samples were the Primatology Center of Rio de Janeiro (CPRJ), the Wild Animal Screening Center - Ibama, Seropédica, the wild animal vivarium of the Carlos Chagas Filho Biophysics Institute within the Federal University of Rio de Janeiro, as well as the Rio Biopark. DNA was extracted from the blood samples for subsequent molecular analysis, using different initiators responsible for amplifying specific genes from the Trypanosomatidae and Anaplasmataceae families, Haemosporida order and the genuses Babesia spp. and Borrelia spp. Eight samples from the CPRJ (8/178) amplified products for the 18S rRNA gene, and after being sequenced, they were aligned and subjected to phylogenetic analysis, with results in seven samples showing sequences with 99% to 100% genetic similarity to Trypanosoma minasense in six animals of the Leontopithecus chrysomelas species (3.9%) and one animal of the Alouatta puruensis species (0.56%), representing the first detection of this agent in these species in the state of Rio de Janeiro. In addition, one sequence expressed 100% genetic similarity to the free-living protozoan Bodo saltans in Leontopithecus chrysomelas.  There was no amplification of the samples when they were submitted to molecular tests for the genes of the other hemoparasites in this study. Nonetheless, frequent monitoring of wild animals, as well as studies involving the relationship between Trypanosoma minasense and its possible vectors and hosts, are relevant, contributing to epidemiological and ecological studies of the agents, vectors and hosts.

COMMITTEE MEMBERS:
Presidente - ***.018.557-** - ADIVALDO HENRIQUE DA FONSECA - UFRRJ
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Externa ao Programa - 3324429 - ANDRESA GUIMARAES - UFRRJExterna à Instituição - CATIA DEJUSTE DE PAULA
Externo à Instituição - PAULO CÉSAR MAGALHÃES MATOS