Banca de DEFESA: DIOGO DOS SANTOS MAIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DIOGO DOS SANTOS MAIA
DATE: 29/09/2023
TIME: 13:30
LOCAL: Videoconferência
TITLE:

Diagnosis of A. platys Dumler et al, 2001 in thrombocytopenic dogs from two mesoregions of the state of Rio de Janeiro

KEY WORDS:

 

Anaplasma platys, prevalence, Rio de Janeiro

PAGES: 69
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Anaplasma platys is a gram-negative, obligatory intracellular bacterium, with tropism for platelets and the etiological agent of Canine Infectious Cyclic Thrombocytopenia (TCIC), a disease distributed worldwide, transmitted by the ixodid tick Rhipicephalus sanguineus sensu lato and commonly associated, as the name implies, with thrombocytopenia in dogs. It is predominant in regions with a subtropical and tropical climate, including Brazil, where it occurs with prevalence rates ranging from 2 to 55%, with reports in all regions of the country. It is known to be a zoonotic agent and its vector has already been described as parasitizing humans in Brazil. Despite its importance, several aspects of the infection are still unknown in many parts of the country. Therefore, this study aimed to determine the prevalence, using hematological and molecular methods, analyze the epidemiological aspects, and evaluate the genetic diversity of A. platys, using the gltA gene, in whole blood samples from naturally infected thrombocytopenic dogs from two mesoregions of the Rio de Janeiro state. Four hundred and four whole blood samples from thrombocytopenic dogs, from the Metropolitana and Sul Fluminense mesoregions, in the state of Rio de Janeiro, were used to perform a complete blood count, blood smear search for tick-borne parasites, molecular diagnosis, genetic sequencing and phylogenetic analysis. Furthermore, the correlation of the variables sex, breed and age of the dogs with positivity for A. platys was verified. Of the samples analyzed, 4.7% (19/404) amplified for the A. platys gltA gene. Of these, 15 were subjected to sequencing and phylogenetic analysis. The sequences showed 99-100% identity with isolados from countries on several continents and formed a unique clade. Anemia, hypochromia, hypoproteinemia and the variable sex (male dogs) were associated with A. platys positivity. The results demonstrated that there is a single genotype of A. platys circulating in the state of Rio de Janeiro and that male dogs are the most infected by the pathogen.

COMMITTEE MEMBERS:
Presidente - 3247559 - BRUNA DE AZEVEDO BAETA
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Externa ao Programa - 3324429 - ANDRESA GUIMARAES - UFRRJExterno à Instituição - JENEVALDO BARBOSA DA SILVA - UFVJM
Externo à Instituição - RENATA FERNANDES FEREIRA DE MORAES - UV