Banca de DEFESA: THEREZA CRISTINA DA COSTA PATRICIO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : THEREZA CRISTINA DA COSTA PATRICIO
DATE: 29/09/2023
TIME: 10:00
LOCAL: https://meet.google.com/rqf-dwot-ipv
TITLE:
Research on Escherichia coli producing beta-lactamase from poultry slaughterhouse circulating water at the stadium in Rio de Janeiro, Brazil
KEY WORDS:
Circulating Water, Escherichia coli, Bacterial Resistance, One Health
PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:
Brazil is the largest exporter and producer of chicken meat in the world. These birds naturally have the presence of bacteria such as Escherichia coli in the gastrointestinal tract that can cause illness or even death of these animals. In order to avoid this, antimicrobials have been added to water and feed as a prophylactic measure and acting as a performance enhancer. However, this may favor the selection of bacteria resistant to existing antimicrobials. Among the mechanisms of resistance in Gram-negative bacteria, the most important is the production of enzymes β-lactamases, especially those of the extended spectrum (ESBL) and AmpC. Knowing that during this process of poultry meat production there is consumption around 5,000 to 21,000 L of water per ton of meat, generating a large amount of effluents along the slaughter chain, it is understood that water becomesis another way of spreading the multidrug-resistant bacteria present in this environment, enhancing transmission between animals, the environment and humans. Thus, the present study aimed to evaluate the quality of circulating water of an avian slaughterhouse and to draw a resistance profile and to detect the presence of isolates of Escherichia coli producing ESBL and AmpC through water samples. The samples were collected in the municipality of São José do Vale do Rio Preto, in the mountainous region of the State of Rio de Janeiro, where the Rio Preto drains the river, at different points of water passage from the poultry slaughterhouse, totaling 16 samples. The Most Probable Number (MPN) technique was used to determine the number of total coliforms and thermotolerant coliforms. The water from the entrance of the slaughterhouse was within the standard required by the legislation, while the treated effluents were ejecting a load that exceeds the maximum quality limit. They were isolated and identified by MALDI-TOF, 76 Escherichia coli. Ampicillin was the least effective antibiotic and Meropenem the most. In addition, 61.8% (47/76) presented resistance to at least one antibiotic, being among these 6.38% (3/47) considered multiresistant (MDR). In relation to the different collection points, it is observed that the entrance has a lower diversity of antibiotic resistance when compared to the exit. Among the isolates analyzed,19.7% (15/76) indicated possible production of ESBL and 6.6% (5/76) of AmpC in the Diffusion Disc Test. In the Double Disc Sensitivity Test (DDST), 10.5% (8/76) were positive 11.8% (9/76) for the Approximation Test with CAZ/CZC and 15.8% (12/76) with CTX/CTC. Only 3 were positive in the three confirmatory tests. Among the 76 isolates, 21% (16/76) were positive for the blaCTX-M-1 gene, and 17.1% (13/76) for the blaCMY-2 gene, where 6.5% (5/76) presented co-production of the gene. When comparing these data with the phenotypic, 68.7% (11/16) had the blaCTX-M-1 gene but did not show ESBL phenotype and 84.6% (11/13) had the blaCMY-2 gene without resistance to cefotaxime. Thus, this study makes it possible to understand the impact of these production facilities as contributors to the phenomenon of antimicrobial resistance through water in a One Health perspective.
COMMITTEE MEMBERS:
Presidente - 1722406 - SHANA DE MATTOS DE OLIVEIRA COELHO
Externa à Instituição - HELENA MARIA NETO FERREIRA DE SOUSA
Externo à Instituição - KAYO CESAR BIANCO FERNANDES - FIOCRUZ