Banca de DEFESA: MARCOS PEREIRA DA SILVA JUNIOR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCOS PEREIRA DA SILVA JUNIOR
DATE: 29/02/2024
TIME: 09:00
LOCAL: Sala 24 do DPA, Anexo I do IV, UFRRJ.
TITLE:

Molecular diagnosis of parasites of birds from captivity and fauna screening or rehabilitation centers in the state of Rio de Janeiro.

KEY WORDS:

Wild birds, bacteria, molecular biology, cytology, protozoa.

PAGES: 70
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The study involving hemoparasites from wild and exotic birds, under human and free-living care, directly assists in the conservation of animals ex situ and in situ, by maintaining the health of animals that are kept in institutions and animals that have suffered anthropogenic actions, since Brazil is home to one of the greatest biodiversity on the planet. Studies to determine the occurrence and diversity of these parasites can clarify the parasite-host relationship and the physical conditions that birds find themselves in when harboring the agent. That said, the objective of this study was to investigate the cytological and molecular occurrence of hemoparasites in birds received by the Wild Animal Rehabilitation Center, the commercial breeding facility and the Environmental Education Park, all located in the western zone of the state of Rio de Janeiro. Whole blood was collected from 104 birds, divided into 55 free-ranging birds and 49 birds kept under human care. From this, blood smears were prepared and molecular analyzes were carried out for agents of the genera Borrelia and Ehrlichia, family Trypanosomatidae and the orders Haemosporida and Piroplasmida. The positivity of hemoparasites in the individuals studied was 13.46% (n=14/104) through the molecular technique, being agents of the orders Haemosporida (11.54%; n=12/104), Piroplasmida (0.96%; n=1/104) and for the Trypanosomatidae family (0.96%; n=1/104). Using the blood smear technique, it was possible to observe the presence of trypomastigote forms in a sample compatible with molecular diagnosis targeting the 24Sα-rDNA gene in a sample obtained from Asio clamator. Merozoites suggestive of Piroplasmida were identified in a blood smear and confirmed using PCR targeting the 18S rDNA gene in free-living Sula leucogaster. With sequencing, the Asio clamator agent had 91.60% similarity to the 24Sα-rDNA gene of Trypanosoma theileri, and two samples with 99.57% similarity to the cytB gene fragment of Haemoproteus (Haemoproteus) paramultipigmentatus in specimens of Columbina talpacoti under rescue conditions. Furthermore, Plasmodium sp. was also observed, with 99.9% identity with the fragment of the cytB gene, in a specimen of Ramphastos toco also from free-living. Plasmodium juxtanucleare was found similar (99% to 100%) to other sequences deposited in GenBank in six individuals of Gallus gallus domesticus, as well as P. juxtanucleare in an individual of Ara ararauna, all under captive conditions. Another finding confirmed after sequencing was P. juxtanucleare in a free-living Rupornis magnirostris individual with 99.57% similarity to the cytB gene sequence deposited in GenBank. Studies involving the frequency of hemoparasites in birds assist in the creation of preventive medicine protocols and quarantine actions for animals that will be kept under human care or that will be reintroduced into the wild, reinforcing the need for knowledge of the agent and its host.

COMMITTEE MEMBERS:
Presidente - 3103528 - CLAUDIA BEZERRA DA SILVA
Interno - 386163 - CARLOS LUIZ MASSARD
Externa à Instituição - IZABELA MESQUITA ARAÚJO - UFRRJ
Externo à Instituição - MATHEUS DIAS CORDEIRO - UFRRJ
Externo à Instituição - RODRIGO GREDILHA DUARTE