Banca de DEFESA: BIANCA AUGUSTO DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BIANCA AUGUSTO DE SOUZA
DATE: 07/03/2024
TIME: 09:00
LOCAL: UFRRJ
TITLE:

Chemical composition of Schinus terebinthifolius Raddi essential oil and in vitro analysis of leishmanicidal activity

KEY WORDS:

visceral leishmaniasis, chemotherapy, essential oils

PAGES: 74
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Leishmaniases are a group of parasitic diseases classified as neglected. They are caused by flagellated protozoa of the genus Leishmania and are transmitted by the bite of infected female phlebotomine vectors. The protozoa of the genus Leishmania are parasites belonging to the Trypanosomatidae family and have two primary evolutionary forms: promastigote, flagellated and extracellular, and amastigote, with flagellodiminute, intracellular. Clinically, the disease manifests itself in 3 forms: visceral, cutaneous, and mucocutaneous, and can affect both humans and animals. Among the relevant species in the epidemiological context, Leishmania infantum causes the most severe form of the disease, called visceral. The drugs currently available are unable to eliminate the parasite and can also generate various side effects for patients, as well as high toxicity and high costs. Natural products are alternative sources for treating various ailments, and several studies have already shown that extracts and essential oils from multiple plant species have leishmanicidal biological activity. The native Brazilian medicinal species, Schinus terebinthifolius Raddi, popularly known as aroeiravermelha, was selected for this study because it has several therapeutic applications and properties already known, such as anti-inflammatory, healing, and antimicrobial properties. Four species genotypes (ARO030-F, ARO071-F, ARO078-F, and ARO136-F) were selected from the germplasm bank of the Agronomy Institute of UFRRJ. Gas chromatography coupled with a mass spectrometer and flame ionization detector (GC-MS and CGDIC) was carried out, where α-pinene (24.79%), limonene (17.26%) and δ-2-care (12.18%) were identified as the majority compounds for ARO030-F. For ARO071-F, α-pinene (36.74%), ρ-cymene (36.22%) and caryophyllene oxide (5.54%). For ARO078-F, Sabinene (20.13%), Terpinen-4-ol (20.11%), and γ-terpinene (13.08%), and for ARO136-F, β-pinene (16.53%), α-terpineol (12.29%) and trans-caryophyllene (12.03%). All the EOs showed leishmanicidal activity, and for ARO030-F, ARO071-F, ARO078-F, and ARO136-F, respectively, the IC50 values were 32.46, 20.52, 41.61, and 32.62 μg/mL on L. infantum promastigote forms. The cell viability of RAW 264.7 murine macrophages was also assessed in the presence of the essential oils, where all the EOs showed low toxicity. The results explained here indicate a promising possibility for using S. terebinthifolius EOs as an alternative chemotherapeutic treatment against the L. infantum parasite and, consequently, against visceral and post-calazar leishmaniasis.

COMMITTEE MEMBERS:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1545840 - LUCIA HELENA PINTO DA SILVA
Externa ao Programa - 3145590 - DANIELA COSENTINO GOMES - UFRRJExterna à Instituição - IVANA CORREA RAMOS LEAL
Externo à Instituição - LUIS ADRIANO ANHOLETO