Action of two Growth Regulators on the Reproductive Efficiency of Dermacentor nitens Engorged Females.
Tick; control; in vitro
In order to evaluate the action of the growth regulators fluazuron and piriproxifen on engorged females of Dermacentor nitens, three “in vitro” methodological tests were performed for each of the two products. Dilutions were performed using the fluazuron and pyriproxyfen raw material diluted in Triton-X 100, N-methylpyrrolidone and acetone, with the serial dilution performed with the diluent containing the last three compounds plus water. Concentrations were in ppm: 4,000; 2,000; 1,000; 500; 250; 125; 62.5, 31.25, 15.62. To perform the immersion the females were grouped in ten, each considered as an experimental unit, and so that the groups had similar weight averages among the concentrations. They were immersed for one minute and then dried and fixed in petri dishes so that the postures were weighed individually. They were conditioned in a heated room with temperature and humidity control for 21 days for weighing the posture, and the quenogins and after further 21 days the percentage of hatching was read. During the first days of incubation the pre-posture period was observed in all the engorged females of the tests until all of them began the laying process. And with all results, the means of hatchability, mortality, reproductive efficiency, reproductive efficiency and nutrient index were calculated separately for each experimental day as well as for the two growth regulators. On observation of the first Fluazuron result showed efficacy above 95% in the concentration of 250ppm with the result of 97.82% and gradually reaching 100% in the last concentration of 4000ppm in others, the value was only found in concentrations of 1000 ppm, being respectively 95.79 and 97.98%, without achieving maximum efficacy. The increase in concentration did not influence the weight of the postures, the weight of the quenogins or the nutritional conversion index of the engorged females. For piriproxifen at the first study, the efficacy was maximal at the concentration of 1000ppm, for the others this level was reached at the concentration of 250ppm. And for this product the influence of the increase of the concentrations in decrease of the average weight of the postures, in the increase of the weight of the kenogins at the end of the posture and in the depletion of the nutritional index was observed clearly. With the results, it is possible to conclude the effectiveness of the two products on the D. nitens tick, with pyriproxyfen showing greater inhibition of the index in the non-parasitic phases and lower concentrations of efficacy.