Banca de DEFESA: ADRIANA BARBOSA DA ROCHA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ADRIANA BARBOSA DA ROCHA SILVA
DATE: 18/11/2024
TIME: 09:00
LOCAL: Plataforma RNP.
TITLE:

Antimicrobial activity of essential oil of Mimosa verrucosa and Illicium verum against planktonic and biomass bacterial cells 

KEY WORDS:

volatile oils, phenylpropanoids, monoterpene, resistance.

 

PAGES: 108
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:

The development of biofilms is a severe health problem, responsible for approximately 80% of microbial infections, including chronic, nosocomial, and medical device-associated infections, which are highly resistant to conventional antibiotics. Essential oils have been evaluated for antimicrobial properties. The study aimed to assess the antimicrobial activity of the essential oils (EOs) of Mimosa verrucosa and Illicium verum against planktonic and sessile cells of Gram-positive and Gram-negative bacteria and evaluate toxicity based on the eukaryotic model of Saccharomyces cerevisiae. Thirty compounds were identified, the main ones being a-pinene (12.6 %), b-pinene (16.7 %) and (E)-caryophyllene (14.2 %) for M. verrucosa, and estragole (4.2 %) and anethole (86.8 %) for I. verum. The results of the minimum inhibitory concentrations (MIC) of the EO of I. verum (29.40 µg/mL) and M. verrucosa (24.89 µg/mL) against planktonic cells showed 99% efficacy against all cells tested (sensitive and resistant S. aureus and E. coli). In sessile cells, the EO of I. verum and M. verrucosa showed respective efficacy against sensitive S. aureus, and all samples were tested. In both analyses, the minimum bactericidal concentration (MBC) test revealed that I. verum caused cell death for sensitive S. aureus and E. coli. However, M. verrucosa only showed bactericidal activity against planktonic cells. The EOs evaluated showed antibacterial and bactericidal activity in the planktonic format. However, only I. verum showed bactericidal activity against sessile cells, indicating its possible potential for treating bacterial biofilms. Considering the expanding resistance to antimicrobials, the EOs tested represent an important therapeutic option, especially against S. aureus and E. coli, which can produce biofilms on various surfaces, becoming a serious public health problem.

COMMITTEE MEMBERS:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1792175 - PATRICIA FAMPA NEGREIROS LIMA
Externa ao Programa - 1609810 - LIDIANE DE CASTRO SOARES - UFRRJExterno ao Programa - 1809123 - ANDRE MARQUES DOS SANTOS - UFRRJExterna à Instituição - NEIDE MARA DE MENEZES EPIFANIO
Externo à Instituição - BRUNO GOMES DE CASTRO
Externo à Instituição - RODRIGO LUIZ FABRI