Banca de DEFESA: PAULA DE ABREU MORAES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : PAULA DE ABREU MORAES
DATA : 28/02/2019
HORA: 10:00
LOCAL: Sala Prof Laerte Grisi-LQEPV
TÍTULO:

Different methods of DNA extraction of fleas ctenocephalides felis felis (Siphonaptera, Pulicidae)

PALAVRAS-CHAVES:

phenol-chloroform; QIAGEN®; boil; salting out; hot-shot; ITS-1

PÁGINAS: 50
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Fleas are important insects from a medical and veterinary point of view, as they are responsiblefortransmittingvariousdiseases.Althoughtherearemorethan2,000species and sub-species worldwide, Ctenocephalides felis felis is one of the most important ectoparasite of dogs and cats currently, mainly because of its geographic distribution, decreased susceptibility to common insecticides. With the advancement of molecular biology,studieswithfleasinvariousareashavebecomeincreasinglyfrequent.Therefore, extractingafleaqualityDNAisextremelyimportantintoday'sscenario.Whensearching theliteratureformethodsofextractingfleaDNA,onecannoticethepredominantuseof commerciallyavailablekits.However,inmanycountriesthecostofthesekitsishighand oftentheiruseisunfeasible.Therefore,thisstudyaimedtosearchforthemainalternative methods of DNA extraction available in the literature and standardize for C. felis felis fleas. The methods used were: phenol-chloroform; boil; salting out; hot-shot; and the DNeasyBlood&TissueKit®(QIAGEN®)kit.TheextractionsweresubmittedtoPCR for amplification, and their efficiency was verified in 1.5% agarose gel. Alternative methodshaveprovedtobeviableoptionsfortheuseofkitsforextractingfleaDNA.

MEMBROS DA BANCA:
Externo à Instituição - ANDRE LUIZ RODRIGUES ROQUE - FIOCRUZ
Externo à Instituição - BRUNA DE AZEVEDO BAETA - UV
Interno - 3701492 - HUARRISSON AZEVEDO SANTOS
Presidente - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Externo ao Programa - 057.185.477-00 - VIVIANE DE SOUZA MAGALHAES - UFRRJ