Banca de DEFESA: ADRIANI DA SILVA CARNEIRO LOPES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ADRIANI DA SILVA CARNEIRO LOPES
DATE: 12/09/2025
TIME: 08:30
LOCAL: Sala 14 do DPA/Anexo 1 IV/IFRRJ
TITLE:

Acaricidal effect of Metarhizium pingshaense associated with Mentha piperita essential oil, Menthol and Menthone on Psoroptes ovis and Dermanyssus gallinae.

KEY WORDS:

Mites, biological control, peppermint, entomopathogenic fungi.

PAGES: 94
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The Psoroptes ovis mite, popularly known as the psoroptic mange mite, sheep mite, rabbit ear mange mite, and others, is a non-burrowing Astigmata mite that commonly causes infestation in livestock. Individuals infested with this ectoparasite tend to suffer from intense itching, crusting in the affected area, alopecia, and the possibility of secondary infections. Meanwhile, Dermanyssus gallinae, popularly known as the red mite, lice mite, and others, is a Mesostigmata mite, with a nesting habit and widespread worldwide distribution, responsible for infestation in poultry, particularly laying hens. Currently, these ectoparasites are controlled with various synthetic acaricides from various classes, such as pyrethroids, organophosphates, macrocyclic lactones, and others. However, the indiscriminate use of these agents has led to resistance to some classes of synthetic acaricides. Therefore, studies are being conducted on alternative control methods, including the use of entomopathogenic fungi and essential oils to control these ectoparasites. Therefore, this study aimed to evaluate the efficacy of a native isolate of Metarhizium pingshaense combined with Mentha piperita essential oil and the isolated compounds menthol and menthone to control the mites P. ovis and D. gallinae. Initially, a compatibility test was performed between the fungal isolate and the essential oil at different concentrations, demonstrating compatibility between them, allowing in vitro mortality assays with the fungus-oil combination to be performed. Individually, M. piperita essential oil, menthol, menthone (0.01 mg/mL), and the fungus exhibited significant acaricidal potential on mites, reducing their survival. In P. ovis, two days after treatment, mortality rates ranged from 21.67% to 100%. On the fourth day, the groups treated with the lowest fungal concentrations showed mortality rates above 75%, differing significantly from the control (40%). Meanwhile, in D. gallinae, after two days of treatment, mortality rates ranged from 51.3% to 64.63%. On the fourth day, even the groups treated with the lowest fungal concentrations showed mortality rates above 71%. When evaluating the combinations, synergistic and antagonistic effects were observed in the treatments for both mites evaluated, depending on the fungal concentration and the specific pairing. SEM analysis of P. ovis revealed fungal mycelial development and cuticular alterations in treated mites, consistent with fungal penetration and activity. However, fungal mycelial development was not observed in D. gallinae, but instead, a variety of cracks in the mites' bodies and detachment of legs and mouthparts were observed. These findings reinforce the potential of integrating entomopathogenic fungi with plant-derived bioactive compounds as part of sustainable control strategies for mites of veterinary importance.

COMMITTEE MEMBERS:
Presidente - 2221855 - PATRICIA SILVA GOLO
Interna - 2860277 - ISABELE DA COSTA ANGELO
Interna - 1282902 - KATIA MARIA FAMADAS
Externa à Instituição - ELEN REGOZINO MUNIZ
Externa à Instituição - MARIANA GUEDES CAMARGO - UFRRJ
Externa à Instituição - EMILY MESQUITA DA SILVA
Externa à Instituição - SIMONE PATRICIA CARNEIRO DE FREITAS