Banca de DEFESA: MARISOL ALVIM GOMEZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARISOL ALVIM GOMEZ
DATA : 27/04/2018
HORA: 09:30
LOCAL: Salão Verde/IV
TÍTULO:

RESISTANCE TO BETA-LACTAMICS AND VANCOMYCIN OF BACTERIAL SPAS WITH IMPORTANCE IN PUBLIC HEALTH ORIGIN OF COMPANION ANIMALS AND VETERINARY HOSPITAL

PALAVRAS-CHAVES:

glycopeptides, One Health, PBP2a

PÁGINAS: 83
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
RESUMO:

The significant narrowing in the relationship between pets and humans over time favors the transmission of bacteria with zoonotic potential directly or through the environment, posing a risk to the health of children and immunosuppressed. Associated with this, the increase in nosocomial infections due to antimicrobial resistance poses a challenge in Human and Veterinary Medicine, highlighting dogs and cats as possible reservoirs of bacterial strains resistant to antimicrobials with importance in public health. The present study aimed to isolate and identify strains of Staphylococcus spp., Enterococcus spp. and E. coli from veterinary hospital environment, diseased and healthy cats and dogs, detect its phenogenotypic resistance to β-lactam and vancomycin., as well as to collect data from bacterial samples with limited phenotypic identification of the Veterinary Microbiological Diagnosis Residence in UFRRJ. Fifty-eight samples of Staphylococcus spp. were identified by biochemistry, PCR and MALDITOF MS as S. aureus (34.48%), S. pseudintermedius (37.93%), S. sciuri (3.45%), S. epidermidis (3.45%), S. intermedius (1.72%), S. luggdunensis (1.72%), S. schleiferi (15.52%) e S. felis (1.72%). A 66.67% agreement was observed with strains identified phenotypically as S. aureus, and 45.24% in S. pseudintermedius. Conflicting results generated by the different techniques are likely to occur due to bias in the biochemical analyzes, the existence of atypical strains and sometimes the insufficient training of the operators, being indicated the complementation by other diagnostic methods in order to characterize correctly the species and the prediction resistance. The blaZ gene was detected in 41.4% of the strains, all phenotypically resistant to penicillin, and mecA gene in 10.34% of the strains, 6.9% by primers described by Murakami et al. (1991), 1.72% by the variant mecA and 1.72% by both primers. A total of 94 strains of E. coli were detected in the study and antibiotic typing revealed that 39.36% belonged to group 2a, 24.47% to group 1e, 10.64% to group 2be, 10.64% to group 2 and 14.89% were classified as penicillinases and cephalosporinases. Expression of ESBLs were phenotypically detected at 20.21% strains, and inducible AmpC beta-lactamase expression in one strain. The blaTEM gene was detected in 24.47% of the strains. All 55 strains of Enterococcus were confirmed by the proteomic technique, 54.55% as E. faecalis, 38.18% as E. faecium and 7.27% as E. hirae, featuring the MALDI-TOF MS an efficient tool to identify this genus. No strain of Enterococcus spp. was resistant to vancomycin. Acinetobacter baumannii and A. pitti were isolated from the hospital floor, respectively exhibiting intermediate resistance to cefotaxime, and to cefotaxime and ceftazidime. Bacterial samples with difficult identification at the genus and species level involved 333 straisn of CoNS, 33 of non-fermenting gram-negative rod strains (except for the genus Pseudomonas) and five of actinomycetes.

MEMBROS DA BANCA:
Externo ao Programa - 1815763 - IRENE DA SILVA COELHO
Externo à Instituição - LIDIANE DE CASTRO SOARES - OUTRO
Presidente - 2212438 - MILIANE MOREIRA SOARES DE SOUZA