Banca de DEFESA: CAROLINA MELO DE SOUZA NUNES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINA MELO DE SOUZA NUNES
DATE: 05/12/2025
TIME: 09:00
LOCAL: DPA sala 14
TITLE:

Molecular characterization of Anaplasma marginale from naturally infected cattle in the state of Rio de Janeiro

KEY WORDS:

Anaplasma marginale; genetic diversity; cattle production.

PAGES: 56
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Bovine anaplasmosis, caused by Anaplasma marginale, is a hemoparasitic disease of major economic and sanitary importance in global livestock production, transmitted primarily by the tick Rhipicephalus microplus. The disease exhibits high genetic diversity, mainly reflected in the surface proteins MSP1a and MSP4, which influence both antigenic variability and the pathogen’s ability to evade the host immune response. This study aimed to assess the prevalence and genetic diversity of A. marginale in cattle from conventional and organic farms in the state of Rio de Janeiro. The evaluation of the farms sought to determine whether inadequate sanitary management, high vector density, and lack of veterinary supervision contribute to the maintenance and spread of the disease. Blood samples (n = 388) were collected across seven municipalities, followed by DNA extraction, molecular amplification of the GAPDH, msp4, and msp1α genes, and analysis of the msp1α tandem repeats. Results showed a 66.8% prevalence of Anaplasma spp. among the samples analyzed, with the identification of eight circulating strains. Genotype E predominated, with one E1 variant, highlighting the genetic diversity of the pathogen in the region. Regarding sanitary management, the positivity rate did not differ statistically. The findings highlight the need to adopt integrated management and control strategies, including continuous epidemiological surveillance and effective vector-control measures, to reduce the prevalence of anaplasmosis and mitigate its impacts on cattle herds.

COMMITTEE MEMBERS:
Presidente - 3247559 - BRUNA DE AZEVEDO BAETA
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Interna - 3103478 - MARISTELA PECKLE PEIXOTO
Externa à Instituição - ANA PAULA MARTINEZ DE ABREU - UV
Externa à Instituição - ERICA CRISTINA ROCHA ROIER - UV