In vitro susceptibility diagnosis of Rhipicephalus microplus (Acari: Ixodidae) to extracts of Chenopodium ambrisiodes and Artemisia vulgaris L.
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The present study aimed to evaluate the in vitro efficacy of Artemisia vulgaris L. and Chenopodium ambrosioides extracts on Rhiphicephalus microplus tick. Initially 95 questionnaires were applied to people who had contact with the rural area. The most commonly used plant was Chenopodium ambrisiodes with 66.7% of citations, mentioned for dog flea control. In order to evaluate in vitro efficacy, teleogins from the R. microplus colony from the UFRRJ Veterinary Experimental Chemotherapy Laboratory, separated into a group of 10, were used in 13 concentrations (10,000 µg / ml, 5,000 µg / ml, 2,500µg). / ml, 1,250µg / ml, 625µg / ml, 312.5µg / ml, 156.25µg / ml, 78,125µg / ml, 39.06µg / ml, 19.53µg / ml) in three extraction forms (Methanol, Dichloromethane Hexane) for each plant Artemisia vulgaris L. and Chenopodium ambrisioides. The hatchability percentage was read to calculate the Product Reproductive Efficiency. The observed efficiencies for both plants were not linear in the progress of increasing concentrations, so the extract with the best results progressed to a second phase. In the first phase the extraction that presented the best result was Artemisia vulgaris L in Hexane, following it to the second phase. In the second phase only the Hexane extracts showed linear results for Artemisia vulgaris L by means of a new Tween use methodology. Thus, it can be concluded that Artemisia vulgaris L has acaricidal potential resulting in extraction by Hexane at a concentration of 40,000 ppm and an efficiency of 85.8.