Banca de DEFESA: MELISSA CRISTINA MORAES FLORENCIO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MELISSA CRISTINA MORAES FLORENCIO
DATA : 12/03/2020
HORA: 00:13
LOCAL: Sala 34 do Instituto de Ciências Biológicas e da Saúde
TÍTULO:

Stomoxys calcitras: establishment of the colony, salivary glands characterization and interaction with pathogens

PALAVRAS-CHAVES:

Stable fly; insect colonies; saliva; mechanical vector.

PÁGINAS: 82
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Stomoxys calcitrans (Diptera), known as stable fly presents great veterinary importance. Males and females feed on blood, a parasitic habit that results in damage to livestock. The consequences of blood feeding include bite stress, which leads to lower weight gain and milk production, and infection with pathogens that S. calcitrans mechanically transmits. The annual damage caused by the stable fly in Brazil is estimated at US $ 340 million. Maintaining this organism under laboratory conditions is a crucial step in establishing research lines under controlled conditions. Insect colonies establishment in the laboratory is important for several studies, ranging from behavior observations to control strategies. This work presents data from S. calcitrans colony that we established in our lab over five months and strategies for breeding this fly in the laboratory. Additionally, we intend to define different actions to better understand the physiological aspects of the vector, as well as its interaction with pathogens in order to better understand the dynamics of mechanical transmission. The saliva composition of hematophagous insects is a crucial evolutionary factor for hematophagy and pathogens have been benefiting from its properties in this relationship. Salivary glands are organs of great importance, both for blood feeding and for the dynamics of pathogen transmission. Therefore, we characterized the salivary glands of S. calcitrans morphologically. The analyzes performed in this work revealed that this organ has merocrine gland characteristic, with polygonal cells presenting large nuclei, besides exhibiting cytoplasm rich in mitochondrial profiles and rough endoplasmic reticulum, characteristics of high protein synthesis activity. Understanding how S. calcitrans interacts with pathogens is an important step to clarify the dynamics of transmission by this fly, and the chosen model was Leishmania, etiological agent of leishmaniasis, diseases with anthropozoonotic characteristics. Leishmania sp. has been able to interact with the S. calcitrans gut, but the mechanisms by which this binding occurs must still be clarified.

MEMBROS DA BANCA:
Interna - 1700427 - YARA PELUSO CID
Interna - 1792175 - PATRICIA FAMPA NEGREIROS LIMA
Externa ao Programa - 2142679 - LEILANE MARIA BARCELLOS NEPOMUCENO
Externa ao Programa - 3103528 - CLAUDIA BEZERRA DA SILVA
Externa à Instituição - LILIAN CRISTINA DE SOUSA OLIVEIRA BATISTA CIRNE - CESVA
Externo à Instituição - WILLY JABLONKA - UFRJ
Externo à Instituição - JACENIR REIS DOS SANTOS MALLET - FIOCRUZ