Banca de DEFESA: RODRIGO GREDILHA DUARTE

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RODRIGO GREDILHA DUARTE
DATE: 24/06/2020
TIME: 14:00
LOCAL: A defesa erá realizada utilizando a plataforma Google Meet
TITLE:

Molecular characterization of new strains of Trypanosoma in birds from Itatiaia National Park and in Mata Mineira Zone (MG), Brazil

KEY WORDS:

Trypanosoma spp., hemoparasites, neotropical birds, Atlantic Forest

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

 

The Atlantic Forest is considered one of the 34 biodiversity hotspots on the planet due to its high species richness and endemism, however the anthropic actions that occur in this biome have contributing to the faunal decline and particularly, hemoparasite infections may be further threat to the birds of this ecosystem. Thus, understanding the diversity, distribution patterns and evolutionary aspects of these parasites in wild bird hosts is important to design actions in conservation programs. In this study, blood samples from 188 wild birds were subjected to conventional PCR genetic analysis using 18S rDNA sequence to identify Trypanosoma spp resulting in a total of 15 positive samples; seven specimens from the Itatiaia National Park (RJ / MG), two in Turdus flavipes and five in T. albicollis, as well as in the capture areas belonging to Zona da Mata Mineira for six individuals of the species Tachyphonus coronatus, one species of Thamnophilus caerulescens and one of Synallaxis spixi. Five clones from each PCR reaction were submitted to sequencing, totaling 15 sequences from each bird specimen evaluated. All 15 sequences obtained from T. albicollis and T. flavipes were identical to each other. In the six specimens of T. coronatus, 5 distinct sequences were observed. All strings of T.caerulescens and S. spixi were identical to each other. Using a strategy of cloning different PCR sequences, it was possible to observe one of the examples of T. coronatus two sequences of different trypanosomatics, or with probable evidence of co-infection. Phylogenetic reconstruction showed the grouping of the seven new strains with parasites of the genus Trypanosoma. The new strains were grouped into two clades. The lines JB03, JB04, JB05, ITA01 and ITA02 are grouped together with the species Trypanosoma bennetti. As the lines JB01 and JB02 are grouped externally to the groups of species T. avium, T. cullicavium and T. corvi. As the new trypanosome lines found in this study are grouped with occurrence lines recorded in Europe, Asia and Africa. According to the evolutionary distance data selected in the present study, we can observe the same genetic strain of Trypanosoma sp. is capable of infecting different species of birds, just as the same bird species can be infected by another strain of Trypanosoma sp. This study, in an unprecedented way, is the first to access a molecular diversity of parasites of the genus Trypanosoma in birds in Brazil. Until then, all studies related to avian trypanosomes were based mainly on reports of occurrence in blood smears of birds in studies aimed at hemoparasites in general. For Brazil, this pioneering study selected possible methods to research a diversity of avian trypanosomes and shows the great potential that exists to be explored in this group of parasites. Due to the results of this study accredited in Brazil, due to its large territorial dimensions and the great biodiversity of hosted birds and insect vectors, it may be one of the richest species of avian trypanosomes in the world.

BANKING MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Interno - 1971595 - BRUNO PEREIRA BERTO
Externa ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Externa ao Programa - 3103528 - CLAUDIA BEZERRA DA SILVA
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ
Externo à Instituição - ALYNNE DA SILVA BARBOSA - UFF
Externo à Instituição - ROBERTO JUNIO PEDROSO DIAS - UFJF