Banca de QUALIFICAÇÃO: DÉBORA MARTINS DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : DÉBORA MARTINS DOS SANTOS
DATE: 05/03/2021
TIME: 14:00
LOCAL: A defesa será realizada utilizando a plataforma Google Meet
TITLE:

Epidemiological aspects of Ehrlichia canis in dogs living in cities with different altitude gradients in the state of Rio de Janeiro

KEY WORDS:

Ehrlichia canis, epidemiology, altitude, geographical distribution. 

PAGES: 63
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

 The canine monocytic ehrlichiosis is one of the most commonly diagnosed diseases in the small animals’ clinic routine. The goal of this study was to analyze the frequency, geographical distribution and epidemiology of Ehrlichia canis in dogs in the cities of Rio de Janeiro state with different altitudes, during the period of one year (from November of 2018 to October of 2019). A total of 456 samples of blood, collected among all the cities studied, were collected for a molecular analyzes. The total DNA was extracted from the blood using a (DNeasy Blood & Tissue kit QIAGEN) extraction kit. Samples were then submitted to a qPCR aiming the 16S rDNA sequence, following protocol previously described in the literature. In the cities of Barra do Piraí e Paracambi (low altitude) the frequency of E. canis positive dogs was of 33% (n= 34/127) and 36,9% (n=38/122), respectively.  In cities at high altitude the frequency of E. canis was 9,7% (n= 10/103) in Petrópolis and 20,4% (n= 21/104) in Teresópolis. In the logistic regression model an association between E. canis positive dogs and altitude was observed (p<0.002). Dogs living in cities at low altitude have a 2,18 higher chance of being infected by E. canis when compared to dogs living in high altitude cities (OR=2,18, p-valor=0,002, IC=1,35-3,54). The variables: access to a urban environment (OR = 1,77, p=0,01, IC=1,12-2,8), Rhipicephalus sanguíneos s.l tick infestation (OR=1,87, p=0,03, IC= 1,05-3,33) and pure breed dogs (OR=1,87, p= 0,018, IC=1,11-3,14) were also associated with the presence of E. canis DNA in the dogs’ blood. The environmental variables such as altitude, access area, tick infestation and dogs with xx breed explain 73% of the occurrence of E. canis in the studied region. These results demonstrate that the altitude has strong influence over the determining causes and frequency of infection by E. canis in dogs in the studied region, as do intrinsic characteristics of the dog.

BANKING MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Externa ao Programa - 1125765 - SANDRA MARIA GOMES THOME
Externo ao Programa - 2688704 - TIAGO MARQUES DOS SANTOS
Externa à Instituição - ERICA CRISTINA ROCHA ROIER - UV
Externa à Instituição - NATHALIE COSTA DA CUNHA - UFF