Banca de DEFESA: LAÍS DE JESUS MORAES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LAÍS DE JESUS MORAES
DATE: 21/05/2021
TIME: 09:00
LOCAL: Meio digital
TITLE:

Evaluation of the prick test as an auxiliary diagnostic method for flea allergy dermatites in dogs

KEY WORDS:

allergy, canines, percutaneous, puntor

Allergic flea bite dermatitis (DAPP) is a common dermatological disease in Brazil, which brings discomfort both to the animal and to the guardian for seeing the animal with intense itching. The diagnosis is usually made through anamnesis and physical examination. These methods are questioned by the owner, now more enlightened and demanding, who seeks a conclusive, objective and quick diagnosis. Some studies have been carried out with the intradermal test (TID) to help diagnose this pathology, but the results differ. An alternative would be the use of the prick test (PT), which has been widely accepted in human medicine as a fast, safe, reliable and low cost method. The aim of the study was to evaluate the prick test as an auxiliary method in the diagnosis of flea allergy dermatitis in dogs. 24 dogs from the Experimental Chemotherapy Laboratory in Veterinary Parasitology (LQEPV) of the beagle breed, male and female, aged one year or more were selected. These animals were divided into two groups: group 1 containing 12 beagles known to have no DAPP and group 2, composed of 12 dogs with clinical diagnosis of DAPP. The animals were physically restrained, placed in lateral decubitus and had the chest area trichotomized and cleaned. Then, the test was carried out. One drop of each solution was used: for the negative control, a glycerin saline solution (50% glycerol - protein stabilizer) and phenolate (0.45% phenol - preservative), positive control (histamine) and the extract of total flea, all in duplicate, and this was inoculated into the dermis with the aid of a Duotip-Test II puntor. The readings were taken after 15 minutes and those papules that presented mean orthogonal diameter equal to or greater than 3 mm of the mean diameter of the negative control were considered positive reactions. Reading 24 and 48 later was also done to assess late reactions. The reactions observed to the flea extract in both groups were weak and considered to be non-reactive. The results suggest that the PT performed in this study had no sensitivity for the diagnosis of DAPP. Therefore, it is necessary to conduct a further study with patients seen in the clinical routine or with other extracts to improve the sensitivity of the technique.

 

 

PAGES: 82
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

Allergic flea bite dermatitis (DAPP) is a common dermatological disease in Brazil, which brings discomfort both to the animal and to the guardian for seeing the animal with intense itching. The diagnosis is usually made through anamnesis and physical examination. These methods are questioned by the owner, now more enlightened and demanding, who seeks a conclusive, objective and quick diagnosis. Some studies have been carried out with the intradermal test (TID) to help diagnose this pathology, but the results differ. An alternative would be the use of the prick test (PT), which has been widely accepted in human medicine as a fast, safe, reliable and low cost method. The aim of the study was to evaluate the prick test as an auxiliary method in the diagnosis of flea allergy dermatitis in dogs. 24 dogs from the Experimental Chemotherapy Laboratory in Veterinary Parasitology (LQEPV) of the beagle breed, male and female, aged one year or more were selected. These animals were divided into two groups: group 1 containing 12 beagles known to have no DAPP and group 2, composed of 12 dogs with clinical diagnosis of DAPP. The animals were physically restrained, placed in lateral decubitus and had the chest area trichotomized and cleaned. Then, the test was carried out. One drop of each solution was used: for the negative control, a glycerin saline solution (50% glycerol - protein stabilizer) and phenolate (0.45% phenol - preservative), positive control (histamine) and the extract of total flea, all in duplicate, and this was inoculated into the dermis with the aid of a Duotip-Test II puntor. The readings were taken after 15 minutes and those papules that presented mean orthogonal diameter equal to or greater than 3 mm of the mean diameter of the negative control were considered positive reactions. Reading 24 and 48 later was also done to assess late reactions. The reactions observed to the flea extract in both groups were weak and considered to be non-reactive. The results suggest that the PT performed in this study had no sensitivity for the diagnosis of DAPP. Therefore, it is necessary to conduct a further study with patients seen in the clinical routine or with other extracts to improve the sensitivity of the technique.

 

BANKING MEMBERS:
Presidente - 4563410 - JULIO ISRAEL FERNANDES
Externo ao Programa - 1120060 - ALEXANDRE JOSE RODRIGUES BENDAS
Externo ao Programa - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Externo à Instituição - ARY ELIAS ABOUD DUTRA - UVA
Externa à Instituição - LILIAN CRISTINA DE SOUSA OLIVEIRA BATISTA CIRNE - CESVA