Banca de DEFESA: HOSANA DAU FERREIRA DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : HOSANA DAU FERREIRA DE SOUZA
DATE: 28/05/2021
TIME: 13:00
LOCAL: Meio Digital
TITLE:

Phenotypic production of extended-spectrum beta-Lactamases by Escherichia coli associated with urinary tract infections in dogs, cats, and humans

KEY WORDS:

Antimicrobial Resistance, One Health, Enterobacterales, ESBL.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:

Antimicrobial resistance is a problem in infections caused by enterobacteria, mainly by strains that produce beta-lactamases, which provide resistance to a wide range of antibiotics. Escherichia coli is a commensal gastrointestinal microbiota bacteria associated with infections in the urinary tract, and strains that produce these enzymes are important pathogens in Veterinary and Human Medicine. Despite the detection of beta-lactamase production being standardized by some committees, such as CLSI (Clinical Laboratory Standard Institute) and BRCast (Brazilian Committee on Antimicrobial Susceptibility Testing), the divergences between them generate extreme difficulty in interlaboratory standardization, generating an crucial barrier to the knowledge of the genuine epidemiology of these enzymes. Thus, the present study aimed to evaluate the resistance profile to β-lactams in E.coli isolated from urine samples from dogs, cats, and humans with urinary tract infections, in addition to researching - through the disc diffusion technique with interpretive reading, the production of ESBL (Extended Spectrum Beta-lactamase), AmpC and Carbapenemases, comparing the detection methods recommended by the normative documents. The study was carried out with 100 strains of E. coli from 273 urine samples from humans, and 97 from 103 samples of dogs and cats, totaling 197 isolates. All isolates had a resistance profile of less than 50% compared to the tested antimicrobials, ampicillin being the least effective, considering both the cutoff points of CLSI and BRCast. A total of 10% (10/100) and 11.16% (22/197) of E.coli from human and animal samples, respectively, were suspected of producing ESBL in the screening test. Among them, 56.25% (18/32) presented suspicion considering the cutoff points of both recommended documents. Of the total of suspected isolates, 81.25% (26/32) were confirmed in the tests - considering at least one normative document. Only 1% (1/100) and 5.15% (5/97) of human and animal samples were AmpC producers, respectively, and among these, four (66.7%, 4/6) were co-producers of ESBL . Finally, two isolates of human origin showed resistance to ertapenem, indicating possible production of carbapenemase. The emergence of multidrug-resistant bacterial strains is worrying due to the aspect of One Health since domestic animals can be reservoirs and transmitting agents for humans and vice versa, where the environment also acts in a potentiating way, contributing significantly to this problem.

BANKING MEMBERS:
Presidente - 1722406 - SHANA DE MATTOS DE OLIVEIRA COELHO
Externa à Instituição - DAYANNE ARAUJO DE MELO - UFRRJ
Externa à Instituição - GREICIANE FRANÇA BRONZATO DE ALMEIDA - UV
Externa à Instituição - INGRID ANNES PEREIRA