Banca de DEFESA: DANIELE DOS SANTOS JULIANO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIELE DOS SANTOS JULIANO
DATE: 04/03/2022
TIME: 09:00
LOCAL: Plataforma Google Meet
TITLE:

Epidemiological analysis of Anaplasma platys in domiciled dogs in different regions from the State of Rio de Janeiro

KEY WORDS:

Epidemiology, dog, molecular techniques, altitude, associated characteristics

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The objective of the present study was to carry out an epidemiological analysis based on the detection of Anaplasma platys through the Real Time PCR technique – also known as qPCR – in blood samples from dogs domiciled in municipalities in the mountain region and low altitude regions located in the Rio de Janeiro state. After detection, the positive samples were submitted to statistical analysis in order to verify the association of epidemiological factors with the presence of A. platys DNA in the blood of dogs from these regions, as well as to elaborate statistical models that can predict variables related to the A. platys infection. Samples were collected from 456 dogs from four municipalities in different regions of the state – Petrópolis and Teresópolis, located in the mountain region – and Barra do Piraí and Paracambi – located in the southern Fluminense region and metropolitan region, respectively. From the blood collection, the genetic material was extracted and submitted to the detection of a specific fragment of the gltA gene, through the application of the Polymerase Chain Reaction – quantitative variation (qPCR) technique. An Epidemiological Questionnaire was developed and applied to dog owners, addressing factors that could be associated with the occurrence of Anaplasma platys infection in animals from the regions selected for the study. Factors such as sex, race, score, size, fur length, age, altitude, zone, climatic period, region of the household, health status of the animal, number of dogs in the household, presence of cats, presence of other domestic animals, presence of rodents, presence of wild animals, presence of cattle, presence of vegetation, street access, use of parasiticides, presence of yard, veterinary assistance, health assessment, housing, castration, flea infestation, infestation by ticks of the Rhipicephalus sanguineus species, infestation by ticks of the genus Amblyomma. Of the 456 samples tested by qPCR, 6.14% were considered positive for Anaplasma platys. Among the 28 animals in which A. platys was detected, 28.6% belonged to Petrópolis and 14.3% to Teresópolis – totaling 42.9% of cases in the mountain region – while 39.3% belonged to Paracambi and 17.8 % to Barra do Piraí – low altitude municipalities, representing 57.1% of occurrences in the regions in question. When analyzing the variables submitted to Logistic Regression, it was observed that dogs residing in low-altitude regions are 3.08 times more likely to be infected by A. platys than animals domiciled in the mountain region. As for the number of dogs in the household, it was found that, in households that have more than one dog, these animals are 3.40 times more likely to be infected with A. platys than a single dog in a household. This study showed that altitude and number of dogs in the home are epidemiological factors of relevance and interest in studies associated with the infection of dogs by A. platys in the municipalities of Petrópolis and Teresópolis – mountain region – and Paracambi and Barra do Piraí – low altitude regions.

BANKING MEMBERS:
Presidente - 386163 - CARLOS LUIZ MASSARD
Externa ao Programa - 3103528 - CLAUDIA BEZERRA DA SILVA
Externo ao Programa - 1636998 - TIAGO MARQUES DOS SANTOS
Externa à Instituição - ALINE MOREIRA DE SOUZA - UFF
Externa à Instituição - ERICA CRISTINA ROCHA ROIER - UV