Banca de DEFESA: HAIKA VICTÓRIA SALES MOREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : HAIKA VICTÓRIA SALES MOREIRA
DATE: 04/03/2022
TIME: 09:00
LOCAL: Google Meet
TITLE:

Metarhizium anisopliae and Beauveria bassiana activity against Aedes aegypti under in vitro and semi-field conditions

KEY WORDS:

Entomopatogenic fungi, semi-field, mosquitoes

PAGES: 61
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:

Aedes aegypti is one of the main diseases vectors in the world and due to resistance cases to insecticides and environmental contamination, entomopathogenic agents are studied as biological control method. It is essential to develop control alternatives under environmental conditions to assess their impacts on mosquito. This work aims to evaluate Metarhizium anisopliae and Beauveria bassiana virulence in vitro and in semi-field on A. aegypti larvae, pupae and adults. L2 larvae, 1 day pupae and adults (Rockefeller strain) up to 7 days were used. The virulence of dry conidia of CG 153, CG 148 and ARSEF 2211 of M. anisopliae and CG 479 of B. bassiana was evaluated, in vitro, on larvae, at 1×10⁵, 10⁶, 10⁷ and 10⁸conidia/mL^-1 concentrations, during seven days. Dry conidia were produced in rice, sieved and sprayed on cups containing 40 mL of sterile dechlorinated water and 10 larvae per cup (N=30). For pupae (N=30), conidial suspensions from the same isolates were prepared at 1×10⁵, 10⁶, 10⁷ and 10⁸ conidia/mL^-1. Conidial suspensions virulence of CG 153 from M. anisopliae and CG 479 from B. bassiana, at 10⁷conidia/mL^-1 were evaluated on larvae and adults under in vitro and semi-field conditions. For larvae, cups containing 100 mL of dechlorinated water and 20 larvae per cup (N=60) were used. In adults in vitro tests, the four concentrations were sprayed on filter papers and placed in cups containing five females and five males. Subsequently, 20 adults were exposed to 10⁷conidia/mL^-1 of CG 153 or CG 479 in semi-field and in vitro simultaneously. Temperature and RH were monitored in semi-field for 15 days. All the isolates reduced larval survival in vitro at 10⁸ conidia/mL^-1, but only CG 153 (χ2=104; P<0.0001) and CG 479 (χ2=69.64; P<0.0001) obteined good results in lower concentrations. In semi-field CG 153 (χ2=247.1; P<0.0001) and CG 479 (χ2=126.2; P<0.0001) reduced larval survival when compared to control group. No isolate reduced significantly pupae survival in vitro. To adults, a significant reduction in CG 153(χ2=25.74; P<0.0001) survival at 10⁷ conidia/mL^-1 and CG 479 (χ2=19.64; P<0.0001) was observed in vitro. There was also a reduction in males survival compared to females at 10⁷ (χ2=13.62; P=0.0002) and 10⁸conidia/mL^-1 (χ2=20.42; P<0.0001) to CG 153 and reduced females survival. in relation to males in 10⁸ conidia/mL^-1 for CG 479(χ2=13.02;P<0.0003). In semi-field, CG 153 (χ2=54.34; P<0.0001) and CG 479 (χ2 =118.9; P<0.0001) reduced survival significantly compared to the control. Females in semi-field had greater survival reduction than males (χ2=6.617; P=0.0101) for CG 479. Therefore, M. anisopliae CG 153 and B. bassiana CG 479 have potential for controlling larvae and adults of A. aegypti in semi-field, which can be used in future field research. This work is the first to evaluate M. anisopliae and B. bassiana virulence against different stages of A. aegypti life cycle in semi-field monitoring climatic conditions capable of interfering in fungal virulence.

BANKING MEMBERS:
Interna - 2221855 - PATRICIA SILVA GOLO
Externa ao Programa - 3241766 - MARIANA GUEDES CAMARGO
Externo à Instituição - EVERTON KORT KAMP - UFG
Externo à Instituição - WENDELL MARCELO DE SOUZA PERINOTTO - UFRB