Banca de DEFESA: LETICIA BAPTISTA PINTO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LETICIA BAPTISTA PINTO
DATE: 18/03/2022
TIME: 14:00
LOCAL: sala virtual - sala 74/IV
TITLE:

Analysis of the Antimicrobial Resistance Profile in Bacteria Isolated from Necropsied Animals at the Federal Rural University of Rio de Janeiro

KEY WORDS:

Keywords: antimicrobial resistance, PBP alteration, ESBL production, vanB, necropsy, One Health.

PAGES: 99
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The emergence and spread of antimicrobial resistance is one of the three main threats to Public Health in the 21st century and must be analyzed in an integrated One Health approach, as it is a health risk shared by people, animals and the environment. Despite understanding the multifactorial origin of antimicrobial resistance, little is known about the contribution of environments aimed at the production, maintenance and care of animals in disseminating this phenomenon. Among these, the necropsy space represents a point of cohesion, being a place of extreme relevance for research and understanding of the circulation of bacterial microbiota and its resistance genes. The present study evaluated the occurrence of superbugs in samples of animals necropsied at the Federal Rural University of Rio de Janeiro, considering the priority criteria established by the World Health Organization (WHO), where Pseudomonas aeruginosa and Acinetobacter baumannii resistant to carbapenems and bacteria of the order Enterobacterales resistant to carbapenems and producing ESBL were classified as level 1 or critical; vancomycin-resistant Enterococcus faecium, methicillin-resistant Staphylococcus aureus and with resistance or intermediate resistance to vancomycin, and Salmonella spp. resistant to fluoroquinolones as level 2 or high priority; and Shigella resistant to fluoroquinolones and S. pneumoniae not susceptible to penicillin as level 3 or medium priority.

Of the 198 samples collected from 45 animals, being 20 companion animals, 20 production animals, and three wild ones, 325 strains were isolated, of which 51,38% (167/325) were Enterobacterales, 31,69% (103/325) Staphylococcus spp., 12,62% (41/325) Enterococcus spp., 2,46% (8/325) Streptococcus spp. and 1,85% (6/325) BGNNF. MALDI-TOF proved to be an efficient tool for bacterial identification, especially in Enterococcus spp. and Enterobacterales. The agreement between biochemical, proteomic and genotypic techniques in identifying Staphylococcus spp. was 80,58%, which confirms the importance of the association between different diagnostic methods for the characterization of this genus, leading to the correct direction of the resistance analysis. 8,74% (9/103) of Staphylococcus spp. showed phenotypic resistance indicative of PBP2a production, with detection of the mecA gene in all strains. Phenotypic resistance to vancomycin was evidenced in E. faecalis, with detection of the vanB gene. In 29,13% (30/103) of Staphylococcus spp. there was detection of the blaZ gene. The ESBL phenotypic research was performed through screening and confirmatory antibiograms recommended by the CLSI. 11,98% (20/167) of enterobacteria showed resistance to beta-lactams in the screening antibiogram and 80% (16/20) of them were positive in the confirmatory test. The search for genes encoding ESBL revealed the presence of bla_SHV in 10,18% (17/167), bla_TEM in 6,59% (11/167) and bla_CTX-M in 4,19% (7/167). There was no detection of carbapenemase-producing strains. No mcr genes were detected. These results reveal species characterized as critical superbugs in the necropsy environment and reinforce the need to monitor these strains in the veterinary environment, not only for the adoption of adequate control and treatment measures for the animals but also for the implementation of safe protocols for the disposal of their carcasses.

BANKING MEMBERS:
Presidente - 2212438 - MILIANE MOREIRA SOARES DE SOUZA
Interna - 1722406 - SHANA DE MATTOS DE OLIVEIRA COELHO
Externa à Instituição - ELEINE KUROKI ANZAI