Banca de QUALIFICAÇÃO: DAYANA PALMEIRA DA SILVA ROSA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : DAYANA PALMEIRA DA SILVA ROSA
DATE: 29/04/2022
TIME: 10:00
LOCAL: https://meet.google.com/bcb-beho-kzn
TITLE:

 

Characterization of circulating hemocytes of Stomoxys calcitrans and its interaction with the trypanosomatid  Herpetomonas muscarum

 

KEY WORDS:

Stomoxys calcitrans, mechanical vectors, hemocytes, Herpetomonas muscarum

PAGES: 47
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Entomologia e Malacologia de Parasitos e Vetores
SUMMARY:

Stomoxys calcitrans is a cosmopolitan and hematophagous dipteran. Its feeding habit is defined as aggressive and persistent, involving different warm-blooded species of hosts, being considered pest of cattle and other animals of economic importance, reducing the weight and milk production in creations. In rearing environments, members of the genus Stomoxys play an important role in the epidemiology of transmissible diseases, being mechanical vectors of several pathogens. Knowing and understanding the cellular components of the fly's immune system and the relationship between the S. calcitrans organism and the pathogens that transmits is essential to clarify the pathways involved in these interactions, identifying the best research targets for the development of control techniques for this livestock pest. Thus, at this work, we counted the number of circulating hemocytes in different evolutionary phases (1410x10⁴ cells/mL in field flies, 630x10⁴ cells/mL in larvae and 164x10⁴ cells/mL in colony individuals), identifying and characterizing circulating hemocytes (prohemocytes, granulocytes, plasmatocytes and oenocytoids). A parasite was found in the hemolymph of Stomoxys calcitrans at concentrations of 33x10⁴ parasites/mL in larvae and 6.5x10⁴ parasites/mL in adults, being also isolated and identified at species level by DNA sequencing, as Herpetomonas muscarum. Its growth curve showed a logarithmic growth phase starting at 24 hours of culture, with a growth peak on the 4th day, with a concentration of 1389x10⁵ parasites/mL after an initial plating of 2x10⁵ parasites/mL. Finally, the interaction of this parasite with the fly isolated gut was evaluated by in vivo and in vitro experiments, demonstrating that it is able to interact and be maintened in the intestine even after a few hours of interaction. In the in vitro tests, 5.8x10⁴ parasites/mL were found after processing the intestines and in the in vivo tests we still found parasites in the intestine after 24 hours of feeding, at a concentration of 5.4x10³ parasites/mL. All the information found here will be important in understanding the physiology of Stomoxys calcitrans, as well as its interaction with pathogens, opening paths for strategies to control this livestock pest.

BANKING MEMBERS:
Presidente - 1792175 - PATRICIA FAMPA NEGREIROS LIMA
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Interno - 1109555 - JAIRO PINHEIRO DA SILVA
Externa ao Programa - 3145590 - DANIELA COSENTINO GOMES