Banca de DEFESA: JOSÉ LUIS RODRÍGUEZ BAUTISTA
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.STUDENT : JOSÉ LUIS RODRÍGUEZ BAUTISTA
DATE: 28/06/2022
TIME: 14:00
LOCAL: VIDEO CONFERÊNCIA
TITLE:
Molecular diagnosis of tick-transmissible bioagents (Acari: Ixodidae) from the state of Minas Gerais, Brazil and the departments of Casanare and Cundinamarca, Colombia.
KEY WORDS:
Molecular Biology, Diagnosis, Ticks, Rickettsia, Hemoparasites
PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:
Ticks and tick-borne pathogens are considered a major global problem for livestock and public health. Tick-borne diseases are highly relevant because many known species of ticks can parasitize and transmit pathogens to animals and humans. The study aimed to evaluate the presence of Rickettsia spp., in addition to a broad group of agents transmitted by ticks collected in rural forest areas in the municipalities of Divino, in the Zona da Mata of Minas Gerais, Brazil, and samples of ticks and blood from cattle from the departments of Casanare and Cundinamarca, Colombia. Ticks are endemic in both countries, and health authorities are very concerned about spotted fever cases in the human population, among other diseases of veterinary interest. A cross-sectional study was carried out in which free-living ticks were collected in forest fragments of livestock and agricultural farms in both countries. Tick collections were carried out in forest fragments, areas of human visitation, and farm animal hosts in the state of Minas Gerais and the department of Casanare, Colombia, in the department of Cundinamarca, Colombia. Ticks from the environment were obtained using techniques such as drag and flannel leggings and by inspection on animals. Ticks were identified using taxonomic keys. DNA was extracted to be tested for the presence of pathogens through the Polymerase Chain Reaction. Two thousand eight hundred thirty ticks were obtained (1,112 in MG, 1,017 in Casanare, and 700 in Cundinamarca). The selected ticks were individually subjected to DNA extraction using the Hot SHOT method. The extracted DNA was tested by a battery of PCR assays targeting the Rickettsia, Borrelia, Ehrlichia, Anaplasma, Babesia, and Theileria genera. The protocol for each reaction was elaborated following as described in the original work for each target. The main tick species identified among the three tick groups were: Amblyomma. sculptum, A. pseudoconcolor, A. auriculatum, A. cajennense patinoi, A. cajennense ss, A. coelebs, A. dissimile, A. naponense, A. ovale, Rhipicephalus sanguineus and Dermacentor nitens. In the group of ticks from Minas Gerais, it was impossible to detect specimens positive to any of the analyzed pathogens. On the other hand, in the group of ticks from both Casanare and Cundinamarca, 49 larvae, 63 nymphs and 16 adults of Casanare and 25 adults of A. ovale, 35 of R. sanguineus, and 20 of A. cajennense s.l. of Cundinamarca as positive to the amplification of a 400bp fragment of the gltA gene. Additionally, a preliminary advance has been reached in confirmation with amplifying fragments of the ompA and htrA genes for further sequencing and identification of Rickettsia species present. Analyzes other pathogens such as Anaplasma spp., Ehrlichia spp., Babesia spp., and Theileria spp. in the Casanare and Cundinamarca tick groups will be done using in-line reverse hybridization (RLBH) using specific probes for some species of the mentioned genera of importance in animal species. Phylogenetic analyzes will be carried out with samples that are positive for each of the pathogens studied.
BANKING MEMBERS:
Interno - 386163 - CARLOS LUIZ MASSARD
Interna - 3103478 - MARISTELA PECKLE PEIXOTO
Externo à Instituição - ADLILTON PACHECO DE OLIVEIRA - UES
Externo à Instituição - HERMES RIBEIRO LUZ - UFMA
Externa à Instituição - IZABELA MESQUITA ARAÚJO - UFRRJ
Externa à Instituição - NATHALIE COSTA DA CUNHA - UFF